Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Homologie
aadA cassette
Attributs du produit
Expected / apparent Molecular Weight of the Antigene: 30 / 30 kDa chloroplast transformation marker
Purification
serum
Immunogène
KLH-conjugated peptide derived from known aadA1 sequences
Indications d'application
1: 3 000 with standard ECL (WB)
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 μL of sterile water
Conseil sur la manipulation
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Stock
-20 °C
Stockage commentaire
store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Kolotilin, Kaldis, Pereira, Laberge, Menassa: "Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields." dans: Biotechnology for biofuels, Vol. 6, Issue 1, pp. 65, (2013) (PubMed).
AadA - aminoglycoside 3adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.