The anti-DYKDDDDK tag affinity gel is composed of the L5 antibody conjugated to an agarose resin, which can be used for purification of proteins expressing the octapeptide, DYKDDDDK. The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins. The binding capacity of the L5 agarose resin is greater than 0.8 mg/mL. The resin can be re-used at least five times (see technical bulletin for regeneration and storage). This affinity gel is also useful for immunoprecipitation of DYKDDDDK-tagged protein.
Purification
The antibody was purified by affinity chromatography.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Buffer
50 % anti-DYKDDDDK-tag (L5) antibody conjugated resin is supplied in 1X PBS and 0.09 % Sodium azide. The volume specified for each catalog number indicates the volume of resin included.
Stock
4 °C
Stockage commentaire
Upon receipt, store between 2°C and 8°C. The unopened product is stable for one year upon arrival.