Buffer
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Dans les tests immunologiques, la précision et la fiabilité des résultats dépendent en grande partie de la sélection minutieuse d'un tampon de blocage approprié. Le rôle des tampons de blocage est crucial pour minimiser la liaison non spécifique, qui est une cause fréquente de bruit de fond dans les essais tels que le Western blotting. La liaison non spécifique est susceptible d'obscurcir les signaux authentiques, ce qui conduit à des interprétations inexactes. Un tampon de blocage bien choisi permet d'améliorer la sensibilité de l'essai en réduisant les interférences de fond, ce qui améliore le rapport signal/bruit. Cela devient particulièrement crucial pour la détection des protéines de faible abondance. Les formules de tampon de blocage d'antibodies-online sont spécifiquement conçues pour diminuer les interactions non spécifiques tout en stabilisant simultanément les anticorps et les antigènes. Cela permet d'obtenir des résultats plus précis et plus durables.
Pour vous aider à choisir le tampon de blocage optimal pour vos essais, nous avons élaboré le guide suivant sur la sélection des tampons de blocage. Ce guide fournit des recommandations et des précautions, assurant la sélection du tampon de blocage le plus efficace pour obtenir des résultats optimaux. Si vous avez d'autres questions sur l'application des tampons, la FAQ en bas de page et notre service clientèle peuvent vous aider.
Blocking Buffer Selection Guide
| Product | Recommended Use | Application Note | Precautions |
|---|---|---|---|
| Blocking Buffer for Fluorescent Western Blotting | Fluorescent WB, ELISA, IF, IHC, Microarray, Multiplex | Ready-to-use solution. Designed for use in fluorescent Western blotting but also useful in other immunoassays with fluorescent labels. | Contains thimerosal, for a thimerosal-free option please see Blocking Buffer (10X) for Fluorescent Western Blotting (Thimerosal Free). |
| Blocking Buffer (2X) for Fluorescent Western Blotting | Fluorescent WB, ELISA, IF, IHC, Microarray, Multiplex | 2X concentrated solution. Designed for use in fluorescent Western blotting but also useful in other immunoassays with fluorescent labels. | Contains thimerosal, for a thimerosal-free option please see Blocking Buffer (10X) for Fluorescent Western Blotting (Thimerosal Free). |
| Blocking Buffer for Fluorescent Western Blotting (Thimerosal Free) 10-Pack | Fluorescent WB, ELISA, IF, IHC, Microarray, Multiplex | 10-pack of ready-to-use solution. Thimerosal-free blocking buffers are often preferred in facilities where safety and environmental impact are significant considerations. | |
| Blocking Buffer (10X) for Fluorescent Western Blotting (Thimerosal Free) | Fluorescent WB, ELISA, IF, IHC, Microarray, Multiplex | 10X concentrated solution. Thimerosal-free blocking buffers are often preferred in facilities where safety and environmental impact are significant considerations. | |
| Normal Goat Serum (NGS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in goat. | |
| Normal Horse Serum (NHS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in horse. | |
| Normal Mouse Serum (NMS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in mouse. | |
| Normal Rabbit Serum (NRS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in rabbit. | |
| Normal Rat Serum (NRS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in rat. | |
| Normal Sheep Serum (NSS) | WB, ELISA, IF, IHC | For use in immunoassays where the primary antibody was produced in sheep. | |
| Bovine Serum Albumin 30% Solution | ELISA, WB, IHC | BSA blocking solutions are preferred with biotin and AP antibody labels. | |
| Bovine Serum Albumin - Fraction V | ELISA, WB, IHC | BSA blocking solutions are preferred with biotin and AP antibody labels. | Since BSA is notoriously difficult to dissolve, you can save time using our Bovine Serum Albumin 30% Solution and dilute further. |
| ELISA Microwell Blocking Buffer with Stabilizer | ELISA | Designed to block microwells coated with antigens, antibodies or other ligands and stabilize the plates for drying. | |
| Blotto Immunoanalytical Grade (Non-Fat Dry Milk) | WB, ELISA | Immunoanalytical grade non-fat dry milk. |
Not suitable for use with biotin-avidin detection due to inherent biotin content. |
| Blotto A Pre-Mixed | WB, ELISA | General purpose blocking agent. | |
| Blotto B Pre-Mixed | WB, ELISA | General purpose blocking agent when phospho-specific antibodies are used. | |
| 10X BBS Fish Gel Concentrate | WB, IHC, IF, Multiplex | Fish gel is less likely to cross-react with antibodies of mammalian origin than conventional blocking reagents such as non-fat dry milk and BSA. | |
| 10X PBS Fish Gel Concentrate | WB, IHC, IF, Multiplex | Fish gel is less likely to cross-react with antibodies of mammalian origin than conventional blocking reagents such as non-fat dry milk and BSA. | For AP antibody labels use 10X TBS Fish Gel Concentrate or 10X BBS Fish Gel Concentrate buffers instead. |
| 10X TBS Fish Gel Concentrate | WB, IHC, IF, Multiplex | Fish gel is less likely to cross-react with antibodies of mammalian origin than conventional blocking reagents such as non-fat dry milk and BSA. | |
| 1X PBS, pH 7.2 Buffer with 1% Casein | WB | Blocking buffers containing casein may provide lower backgrounds than buffers containing non-fat milk or BSA. Casein is recommended for applications using biotin-avidin complexes. | For AP antibody labels use 1X TBS, pH 7.8 Buffer with 1% Casein instead. |
| 1X TBS, pH 7.8 Buffer with 1% Casein | WB | Blocking buffers containing casein may provide lower backgrounds than buffers containing non-fat milk or BSA. Casein is recommended for applications using biotin-avidin complexes. | |
| Blocking Buffer Sampler Kit | Fluorescent WB, WB, ELISA, IF, IHC, Microarray, Multiplex | Set of different blocking buffers for all sorts of immunoassays. |
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