GFP anticorps

N° du produit ABIN100085

Détail du produit anti-GFP anticorps

Highlights:

• Anticorps polyclonal non conjugué de la GFP pour une détection fiable de la GFP et de ses variantes.
• Validé pour la microscopie à fluorescence, ELISA, Western Blotting.
• Anticorps GFP de haute qualité, cité dans plus de 177 références PubMed.
• Disponible en quantités de 10 µl et 100 µl.

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Fonction: Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP.

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

Réactivité croisée (Details): wt, rGFP, eGFP

Attributs du produit: Anti-GFP is designed to detect GFP and its variants.

Purification: GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogentype: Recombinant

Isotype: IgG

Informations sur le produit:

À quoi peut servir l'anticorps GFP ABIN100085 ? Cet anticorps polyclonal non conjugué de la GFP a été développé pour détecter la GFP et ses dérivés. Il détecte de manière fiable les variantes recombinantes et améliorées de la protéine en plus de la GFP de type sauvage. L'anticorps GFP de chèvre a été validé pour le Western blot, la microscopie à immunofluorescence et l'ELISA. Utilisez notre anticorps GFP pour détecter la GFP par liaison directe à l'antigène avec un ELISA de type sandwich ou de capture. Détectez la GFP par microscopie à fluorescence dans des systèmes d'expression procaryotes et eucaryotes, par exemple dans des cellules E.coli ou CHO. Utilisez un conjugué de fluorochrome à cette fin, ce qui conduit à nouveau à une amélioration significative du signal fourni par la fluorescence naturelle de la GFP. Pour l'immunoblotting, utilisez un anticorps polyclonal GFP conjugué à la peroxydase ou à la phosphatase alcaline pour détecter la GFP ou les protéines contenant la GFP sur les Western blots.

Quelles sont les données de validation disponibles pour cet anticorps GFP ? L'anticorps GFP a été utilisé dans 177 publications à ce jour, qui sont énumérées ci-dessous et peuvent être consultées sur Pubmed. 15 Les images du produit illustrent les performances en western blot et en microscopie de fluorescence.

Quelle est la fonction de la GFP ? La GFP, la protéine fluorescente verte, est une protéine fluorescente initialement isolée de la méduse Aequorea victoria. Elle émet une lumière verte de longueur d'onde 509nm lorsqu'elle est excitée par une lumière bleue. La GFP est largement utilisée dans la recherche cellulaire. Elle permet de visualiser les protéines marquées avec la GFP dans les cellules vivantes, ce qui permet un large éventail d'investigations.

Information d'application

Indications d'application: ELISA : 1:10,000 - 1:30,000
IF Microscopy : 1:500
Western Blot : 1:1,000 - 1:10,000
Immunohistochemistry: 1:200 - 1:1,000
ImmunoPrecipitation: Yes

Commentaires:

This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (ABIN129564) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (ABIN964537).
Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.
For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.08 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store GFP antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-GFP (ABIN100085)

Lapraz, Boutres, Fixary-Schuster, De Queiroz, Plaçais, Cerezo, Besse, Préat, Noselli: "Asymmetric activity of NetrinB controls laterality of the Drosophila brain." dans: Nature communications, Vol. 14, Issue 1, pp. 1052, (2023) (PubMed).

Cone, Hurwitz, Lee, Yuan, Zhou, Li, Meckes: "Alix and Syntenin-1 direct amyloid precursor protein trafficking into extracellular vesicles." dans: BMC molecular and cell biology, Vol. 21, Issue 1, pp. 58, (2020) (PubMed).

Kim, Kim, Choi, Lee, Lee, Im, Shin, Kim, Hong, Kim, Kim, Sung: "Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients." dans: Translational neurodegeneration, Vol. 9, Issue 1, pp. 23, (2020) (PubMed).

Saegusa, Hosoya, Nishiyama, Saeki, Fujimoto, Okano, Fujioka, Ogawa: "Low-dose rapamycin-induced autophagy in cochlear outer sulcus cells." dans: Laryngoscope investigative otolaryngology, Vol. 5, Issue 3, pp. 520-528, (2020) (PubMed).

Nkosi, Sun, Duke, Patel, Surapaneni, Singh, Meckes: "Epstein-Barr Virus LMP1 Promotes Syntenin-1- and Hrs-Induced Extracellular Vesicle Formation for Its Own Secretion To Increase Cell Proliferation and Migration." dans: mBio, Vol. 11, Issue 3, (2020) (PubMed).

Rayaprolu, Gao, Xiao, Ramesha, Weinstock, Shah, Duong, Dammer, Webster, Lah, Wood, Betarbet, Levey, Seyfried, Rangaraju: "Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease." dans: Molecular neurodegeneration, Vol. 15, Issue 1, pp. 28, (2020) (PubMed).

Lee, Hanchate, Kondoh, Tong, Kuang, Spray, Ye, Buck: "A psychological stressor conveyed by appetite-linked neurons." dans: Science advances, Vol. 6, Issue 12, pp. eaay5366, (2020) (PubMed).

Chen, Mohammad, Pazdernik, Huang, Bowman, Tycksen, Schedl: "GLP-1 Notch-LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1." dans: PLoS genetics, Vol. 16, Issue 3, pp. e1008650, (2020) (PubMed).

Li, Bao, Luo, Yoan, Sullivan, Quintanilla, Wickersham, Lazarus, Shin, Song: "Supramammillary nucleus synchronizes with dentate gyrus to regulate spatial memory retrieval through glutamate release." dans: eLife, Vol. 9, (2020) (PubMed).

Schäfer, Kaisler, Scheller, Kirchhoff, Haghikia, Faissner: "Conditional Deletion of LRP1 Leads to Progressive Loss of Recombined NG2-Expressing Oligodendrocyte Precursor Cells in a Novel Mouse Model." dans: Cells, Vol. 8, Issue 12, (2020) (PubMed).

Lehner, Spitzer, Gehwolf, Wagner, Weissenbacher, Deininger, Emmanuel, Wichlas, Tempfer, Traweger: "Tenophages: a novel macrophage-like tendon cell population expressing CX3CL1 and CX3CR1." dans: Disease models & mechanisms, Vol. 12, Issue 12, (2020) (PubMed).

Ganjam, Bolte, Matschke, Neitemeier, Dolga, Höllerhage, Höglinger, Adamczyk, Decher, Oertel, Culmsee: "Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons." dans: Cell death & disease, Vol. 10, Issue 11, pp. 865, (2020) (PubMed).

Bongartz, Gille, Hessenkemper, Mandel, Lewitzky, Feller, Schaper: "The multi-site docking protein Grb2-associated binder 1 (Gab1) enhances interleukin-6-induced MAPK-pathway activation in an SHP2-, Grb2-, and time-dependent manner." dans: Cell communication and signaling : CCS, Vol. 17, Issue 1, pp. 135, (2020) (PubMed).

Javier-Torrent, Marco, Rocandio, Pons-Vizcarra, Janes, Lackmann, Egea, Saura: "Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA." dans: eLife, Vol. 8, (2020) (PubMed).

Shibata, Iseda, Mitsuhashi, Oka, Shindo, Moritoki, Nagai, Otsubo, Inoue, Sasaki, Akazawa, Takahashi, Schalek, Lichtman, Okano: "Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy." dans: Frontiers in neural circuits, Vol. 13, pp. 29, (2020) (PubMed).

Turnham, Smith, Kenerson, Omar, Golkowski, Garcia, Bauer, Lau, Sullivan, Langeberg, Ong, Riehle, Yeung, Scott: "An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma." dans: eLife, Vol. 8, (2020) (PubMed).

Zibetti, Liu, Wan, Qian, Blackshaw: "Epigenomic profiling of retinal progenitors reveals LHX2 is required for developmental regulation of open chromatin." dans: Communications biology, Vol. 2, pp. 142, (2020) (PubMed).

Kockel, Griffin, Ahmed, Fidelak, Rajan, Gould, Haigney, Ralston, Tercek, Galligani, Rao, Huq, Bhargava, Dooner, Lemmerman, Malusa, Nguyen, Chung, Gregory, Kuwana, Regenold, Wei, Ashton, Dickinson et al.: "An Interscholastic Network To Generate LexA Enhancer Trap Lines in Drosophila. ..." dans: G3 (Bethesda, Md.), Vol. 9, Issue 7, pp. 2097-2106, (2020) (PubMed).

van der Helm, Barnhoorn, de Jonge-Muller, Molendijk, Hawinkels, Coenraad, van Hoek, Verspaget: "Local but not systemic administration of mesenchymal stromal cells ameliorates fibrogenesis in regenerating livers." dans: Journal of cellular and molecular medicine, Vol. 23, Issue 9, pp. 6238-6250, (2020) (PubMed).

Crowther, Lim, Asrican, Albright, Wooten, Yeh, Bao, Cerri, Hu, Ian Shih, Asokan, Song: "An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus." dans: Stem cell reports, Vol. 10, Issue 3, pp. 1146-1159, (2019) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP (GFP Produits)

Synonymes: anticorps green fluorescent protein, anticorps gfp

Sujet: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.

UniProt: P42212