KLH anticorps

N° du produit ABIN103377

Détail du produit anti-KLH anticorps

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivité: Keyhole Limpet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KLH est non-conjugé

Application: ELISA, Western Blotting (WB)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using KLH coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Stérilité: Sterile filtered

Immunogène: KLH (Keyhole Limpet Hemocyanin)
Immunogentype:Native

Isotype: IgG

Information d'application

Indications d'application: Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-KLH (ABIN103377)

Datta, Silverman, Phipps, Hiraragi, Ratner, Lairmore: "Human T-lymphotropic virus type-1 p30 alters cell cycle G2 regulation of T lymphocytes to enhance cell survival." dans: Retrovirology, Vol. 4, pp. 49, (2007) (PubMed).

Détails sur KLH

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Produits

Sujet: KLH Antibody detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: Keyhole Limpet Hemocyanin, KLH, Anti-KLH antibody