KLH anticorps (HRP)

N° du produit ABIN103381

Détail du produit anti-KLH anticorps (HRP)

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivité: Keyhole Limpet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KLH est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: KLH (Keyhole Limpet Hemocyanin
Immunogentype:Native

Isotype: IgG

Information d'application

Indications d'application: Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 is suggested for this product.  Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Gentamicin sulfate

Conseil sur la manipulation: Do NOT add Sodium Azide!

Stock: 4 °C

Détails sur KLH

Antigène: KLH (Keyhole Limpet Hemocyanin (KLH))

Abstract: KLH Produits

Sujet: KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: HRP, Keyhole Limpet Hemocyanin