beta Actin anticorps (N-Term)

N° du produit ABIN1043747

Détail du produit anti-beta Actin anticorps

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Lapin, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. beta Actin antibody is specific for human and mouse ß Actin protein. Expected to cross-react with a wide range of species due to sequence homology. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.

Attributs du produit: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Stérilité: Sterile filtered

Immunogène: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Immunogen Type: Peptide

Clone: 3H2-E4-E12

Isotype: IgG2a lambda

Information d'application

Indications d'application: Anti-beta Actin antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Commentaires:

Gene Name: Actb

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-beta Actin (ABIN1043747)

Carvajal, Vallejos, Walters, Contreras, Hertz, Olivares, Cáceres, Pino, Letelier, Thompson, López-Lastra: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." dans: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: beta Actin (ACTB Produits)

Synonymes: anticorps BRWS1, anticorps PS1TP5BP1, anticorps ACTA1, anticorps Actx, anticorps E430023M04Rik, anticorps beta-actin, anticorps ps1tp5bp1, anticorps act-2, anticorps actb, anticorps B-actin, anticorps LOC100174883, anticorps LOC100304816, anticorps A, anticorps ACT5C, anticorps Ac5C, anticorps Act, anticorps Act-5C, anticorps Act5, anticorps Act5c, anticorps Actin, anticorps Actin/BAP47, anticorps Actin5C, anticorps BAP47, anticorps Bap47, anticorps CG4027, anticorps Dmel\\CG4027, anticorps M32055, anticorps T11, anticorps act, anticorps act 5C, anticorps act5C, anticorps actin, anticorps actin5C, anticorps anon-EST:fe2D2, anticorps beta-actin/Bap47, anticorps cyt5C, anticorps l(1)G0009, anticorps l(1)G0010, anticorps l(1)G0025, anticorps l(1)G0079, anticorps l(1)G0117, anticorps l(1)G0177, anticorps l(1)G0245, anticorps l(1)G0330, anticorps l(1)G0420, anticorps l(1)G0486, anticorps Bact, anticorps ACT-5, anticorps ACTB, anticorps B-ACTZF, anticorps actba, anticorps bact, anticorps bactin1, anticorps bactzf, anticorps wu:fd18f05, anticorps A26C1B, anticorps POTE2alpha, anticorps POTEACTIN, anticorps Beta-actin, anticorps actin beta, anticorps actin, beta, anticorps cytoplasmic actin OlCA1, anticorps beta-actin, anticorps Actin 5C, anticorps actin gamma 1, anticorps actin, beta 1, anticorps actin, beta L homeolog, anticorps beta actin, anticorps POTE ankyrin domain family member F, anticorps actin, beta like 2, anticorps actin, cytoplasmic 1, anticorps ACTB, anticorps Actb, anticorps actb, anticorps LOC100174883, anticorps LOC100304816, anticorps Act5C, anticorps ACTG1, anticorps actb1, anticorps actb.L, anticorps LOC100136352, anticorps POTEF, anticorps ACTBL2, anticorps LOC109073280

Sujet: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

ID gène: 11461

NCBI Accession: NP_031419

UniProt: P60710

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction