AKT1 anticorps (pThr308) (Biotin)

N° du produit ABIN1043753

N° du produit (Fournisseur): 200-306-269

Il existe 4+ publications pour ce produit. L’anticorps anti-AKT1 Monoclonal Souris est utilisé pour la détection de AKT1 dans des échantillons de Humain, Souris, Rat et Singe. Il a été validé pour WB, IHC, ELISA, FACS, IP et DB.

Aperçu rapide pour AKT1 anticorps (pThr308) (Biotin) (ABIN1043753)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Dot Blot (DB)

Clone: 18F3-H11

Détail du produit anti-AKT1 anticorps (Biotin)

Épitope: pThr308

N° du produit (Fournisseur): 200-306-269

Fournisseur: Rockland

Fonction: AKT phospho T308 Antibody Biotin Conjugated

Réactivité croisée (Details): This antibody is specific for human and mouse AKT protein phosphorylated at T308.

Attributs du produit: Synonyms: mouse anti-AKT pT308 Biotin conjugated Antibody, Biotin conjugated mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated

Purification: Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography.

Immunogène:

Immunogen: Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Labeling Ratio: 10-20

Information d'application

Indications d'application:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 20 μg/mL

Application Note: Biotin Conjugated Anti-AKT pT308 is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 50μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-AKT1 (Biotin) (ABIN1043753)

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" dans: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, (2001) (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." dans: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, (2001) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, (1987) (PubMed).

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1

Sujet: Background: Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.

ID gène: 207, 62241011

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg