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JAG1 anticorps (Internal Region)

JAG1 Reactivité: Humain, Souris WB, ELISA, IHC, FM Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN129524
  • Antigène Voir toutes JAG1 Anticorps
    JAG1 (Jagged 1 (JAG1))
    Épitope
    • 12
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 85
    • 42
    • 36
    • 1
    Humain, Souris
    Hôte
    • 73
    • 15
    • 3
    • 3
    • 1
    Lapin
    Clonalité
    • 70
    • 25
    Polyclonal
    Conjugué
    • 42
    • 9
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp JAG1 est non-conjugé
    Application
    • 63
    • 33
    • 27
    • 16
    • 11
    • 11
    • 10
    • 9
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
    Specificité
    This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
    Attributs du produit
    Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.
    Purification
    purified
    Stérilité
    Sterile filtered
    Immunogène
    This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.
    Immunogen Type: Peptide
    Isotype
    IgG
  • Indications d'application
    This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kDa band in human brain and kidney lysates.
    Commentaires

    Gene Name: JAG1

    Restrictions
    For Research Use only
  • Validation #103702 (Western Blotting)
    'Independent Validation' signe
    by
    AG Pancreatic Development and Stem cell differentiation, Universitätsklinikum Ulm
    No.
    #103702
    Date
    16.06.2019
    Antigène
    JAG1
    Numéro du lot
    28285
    Application validée
    Western Blotting
    Contrôle positif

    human pluripotent stem cells (day 0)

    pancreatic progenitor cells (day 14)

    Contrôle négative

    definite endoderm cells (day 4)

    pancreatic endoderm cells (day 10)

    Conclusion

    ABIN1043767 reveals a protein band of the expected MW and some weaker extraneous bands in cell lysates of human pluripotent stem cells and pancreatic progenitor cells.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN1043767
    Anticorps secondaire
    donkey anti-rabbit HRP-conjugated antibody (GE Healthcare, NA9310V)
    Full Protocol
    • Grow HUES8 in mTeSR1 (STEMCELL Technologies, 85850) at 37°C and 5% O2, 5% CO2 in 2ml in a 6-well plate to 90% confluency.
    • Harvest cells using TrypLE (ThermoFisher Scientific, 12604013) following the manufacturer´s instructions.
    • Lyse 2x106 cells in 30µl per well cold RIPA buffer (50mM Tris-HCl pH 8.0, 150mM NaCl, 0.1% SDS, 0.5% deoxycholate, 1% TritonX 100 in ddH2O) supplemented with 1mM PMSF and 1x protease inhibitor (Roche, 11836170001) for 30min on ice with 3x vortexing in 1.5ml microcentrifuge tubes.
    • Centrifuge tubes at 10000xg for 8min at 4°C.
    • Transfer supernatant to a new 1.5ml microcentrifuge tube and store at -80°C.
    • Determine total protein content of the lysates using a Bradford assay (Bio-Rad, 500-0006).
    • Denature 50µg of total protein for 5min at 95°C in 30µl 1x Laemmli SDS sample buffer and subsequently separate them on a denaturing 7.5% polyacrylamide gel (7.5% Acrylamide, 0.375M Trís-HCl (pH8.8), 0.1% SDS, 0.1% APS, 0.1% TEMED) for about 90min at 120V.
    • Transfer proteins onto a PVDF membrane (Sigma Aldrich, IPVH00010) with transfer buffer (5.27g Tris, 2.93 g glycerine, 200 ml methanol, fill to 1l ddH2O) in a semidry western blotting system for 75min at 80mA/gel.
    • Check transfer of the separated proteins by Ponceau S staining.
    • Rinse membrane with water.
    • Wash membrane for 5min with TBST (TBS, 0.02% Tween20).
    • Block the membrane with 20ml blocking buffer (TBST, 5% milk) on a shaker for 1h at RT.
    • Rinse membrane 3x with TBST.
    • Wash membrane on a shaker 3x for 5min with TBST.
    • Shrink-wrap and incubate membrane with primary
      • rabbit anti-JAG1 antibody (antibodies-online, ABIN1043767, lot 28285) diluted 1:1000 and 1:10000 respectively in blocking buffer ON at 4°C or
      • mouse anti-beta actin antibody (Sigma Aldrich, A1978) diluted 1:2000 in blocking buffer at RT for 1h.
    • Wash membrane 3x for 5min with TBST.
    • Incubate membrane with secondary
      • donkey anti-rabbit HRP-conjugated antibody (GE Healthcare, NA9310V) diluted 1:5000 in TBST containing 1% milk for 1h at RT or
      • donkey anti-mouse HRP-conjugated antibody (GE Healthcare, NA9340V) diluted 1:5000 in TBST containing 1% milk for 1h at RT.
    • Wash membrane 3x for 5min with TBST.
    • Reveal protein bands using ECL solution (ThermoScientific, 34076) on a Fusion SL (Vilber) chemiluminescence system. Exposure times for the 1:1000 and 1:10000 dilutions of ABIN1043767 were 30sec and 5min respectively.
    Notes
    • The bands marked by the arrow in the western blot image using ABIN1043767 at a 1:1000 dilution seem to be specific, as JAG1 showed highest transcriptomic levels at pancreatic progenitor stage (lanes 7 and 8), followed by pluripotent stem cell state (lane 2). Although the immunoblot with ABIN1043767 results in some unspecific bands, the expected band height fits nicely (75kDa). Higher MW bands might potentially represent precursor proteins detectable in all samples but pancreatic progenitor stage samples. In principle specific bands are also detectable with ABIN1043767 at a 1:10000 dilution, although exposition time was very long.

    • ABIN1043767 was also tested in IHC on 4-5µm FFPE sections of primary human kidney cancer tissue. Epitope retrieval was carried out using Tris-EDTA buffer at pH9.0 (Zytomed, ZUC029-500), EDTA at pH8.0 (Leica, RE7116), or citrate buffer at pH6.1 (Agilent, S169984-2) for 20min in a decloaking chamber. Antigen retrieval at pH8.0 looks ok, assuming a broad expression pattern. However, detection would be rather expected in fewer tubular cells. No staining was observed in glomeruli. Cytoplasmic staining in tubuli is revealed at higher magnification. Note that antigen retrieval influences the staining pattern. All in all, staining needs to be validated for example in protein ablation assays.

  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Date de péremption
    12 months
  • Boareto, Jolly, Goldman, Pietilä, Mani, Sengupta, Ben-Jacob, Levine, Onuchic: "Notch-Jagged signalling can give rise to clusters of cells exhibiting a hybrid epithelial/mesenchymal phenotype." dans: Journal of the Royal Society, Interface, Vol. 13, Issue 118, (2017) (PubMed).

    Yabe, Matsumoto, Tsurumoto, Shindo: "Immunohistological localization of Notch receptors and their ligands Delta and Jagged in synovial tissues of rheumatoid arthritis." dans: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, Vol. 10, Issue 6, pp. 589-94, (2005) (PubMed).

  • Antigène
    JAG1 (Jagged 1 (JAG1))
    Autre désignation
    Jagged 1 (JAG1 Produits)
    Synonymes
    anticorps AGS, anticorps AHD, anticorps AWS, anticorps CD339, anticorps HJ1, anticorps JAGL1, anticorps ABE2, anticorps Gsfabe2, anticorps Htu, anticorps Ozz, anticorps Ser-1, anticorps JAG1, anticorps jag3, anticorps cb243, anticorps jag1, anticorps sb:cb243, anticorps serrateC, anticorps jagged1, anticorps serrate, anticorps serrate-1, anticorps x-serrate-1, anticorps jagged 1, anticorps jagged 1b, anticorps jagged 1a, anticorps jagged 1 L homeolog, anticorps JAG1, anticorps Jag1, anticorps jag1, anticorps jag1b, anticorps jag1a, anticorps jag1.L
    Sujet
    Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.
    Synonyms: Ser 1 antibody, AGS antibody, AHD antibody, AWS antibody, CD 339 antibody, CD339 antibody, CD339 antigen antibody, Headturner antibody, HJ1 antibody, Htu antibody
    ID gène
    182, 4557679
    UniProt
    P78504
    Pathways
    Signalisation Notch, Stem Cell Maintenance
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