AKT2 anticorps (Internal Region)

N° du produit ABIN1043774

N° du produit (Fournisseur): 200-501-e71

Détail du produit anti-AKT2 anticorps

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Épitope: Internal Region

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

N° du produit (Fournisseur): 200-501-e71

Fournisseur: Rockland

Fonction: AKT2 Antibody

Réactivité croisée (Details): Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein.

Attributs du produit: Synonyms: rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Immunogen Type: Conjugated Peptide

Clone: 16G11-E8

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: 20 μg/mL

Application Note: Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Western Blot Dilution: 1:500- 1:2000

Immunoprecipitation Dilution: 20 μg/mL

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-AKT2 (ABIN1043774)

Altomare, Kozak, Sonoda, Testa: "Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene." dans: Cytogenetics and cell genetics, Vol. 74, Issue 4, pp. 248-51, (1997) (PubMed).

Détails sur AKT2

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Autre désignation: AKT2 (AKT2 Produits)

Sujet: Background: AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

ID gène: 208

NCBI Accession: NP_001317440

UniProt: P31751

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling