IL-18 anticorps (AA 1-157)

N° du produit ABIN964783

Détail du produit anti-IL-18 anticorps

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Épitope: AA 1-157

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-18 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Radioimmunoassay (RIA), Neutralization (Neut)

Specificité: This is an IgG preparation of whole rabbit serum purified by protein A chromatography using a low endotoxin methodology. This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions. A control of similarly diluted LOW ENDOTOXIN CONTROL RABBIT IgG (code # 011-001-297) is recommended.

Attributs du produit: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64% aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91% aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.

Stérilité: Sterile filtered

Immunogène: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application: This IgG fraction antibody of anti-Mouse IL-18 has been tested for use in neutralizations, ELISA, immunohistochem-istry and immunoblotting. Although untested, this reagent may be useful for radioimmunoassays, flow cytometry and immunoprecip-itation. It recognizes the 18,000 MW mature (active) IL-18. Reactivity in other immunoassays is unknown.

Commentaires:

Gene Name: IL18

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-IL-18 (ABIN964783)

Quach, Song, Guo, Li, Maazi, Fung, Sands, OConnell, Restrepo-Vassalli, Chai, Nemecio, Punj, Akbari, Idos, Mumenthaler, Wu, Martin, Hagiya, Hicks, Cui, Liang: "A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice." dans: Nature communications, Vol. 10, Issue 1, pp. 5681, (2020) (PubMed).

Leal-Lasarte, Franco, Labrador-Garrido, Pozo, Roodveldt: "Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 31, Issue 7, pp. 2797-2816, (2017) (PubMed).

Sakamuri, Valente, Siddesha, Delafontaine, Siebenlist, Gardner, Bysani: "TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis." dans: Molecular and cellular endocrinology, Vol. 429, pp. 84-92, (2016) (PubMed).

Détails sur IL-18

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Autre désignation: IL-18 (IL18 Produits)

Synonymes: anticorps Igif, anticorps Il-18, anticorps IL-18, anticorps IGIF, anticorps ChIL-18, anticorps IL-1g, anticorps IL1F4, anticorps IL18, anticorps interleukin 18, anticorps Il18, anticorps IL18

Sujet: Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64% aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91% aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.
Synonyms: Iboctadekin antibody, IFN gamma inducing factor antibody, IGIF antibody, IL 1 gamma antibody, IL 18 antibody, IL 1g antibody, IL-1F4, IL 18, Interleukin 18, IL18, Interleukin18, IL1 F4, IL1F4

ID gène: 16173

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome