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Myc Tag anticorps

WB, IP, ELISA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN104383
  • Antigène Tous les produits Myc Tag
    Myc Tag
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 75
    • 48
    • 6
    • 5
    • 5
    • 5
    • 1
    • 1
    Lapin
    Clonalité
    • 77
    • 57
    • 10
    Polyclonal
    Conjugué
    • 66
    • 13
    • 10
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Myc Tag est non-conjugé
    Application
    • 108
    • 52
    • 41
    • 36
    • 25
    • 23
    • 20
    • 18
    • 15
    • 11
    • 11
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Séquence
    EQKLISEEDL
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Immunogène
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide.  The sequence corresponds to amino acids 410-419 of human c-Myc.
    Isotype
    IgG
  • Indications d'application
    Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene.   As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope.  The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins.  This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
  • Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." dans: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

    Fujikawa, Matsumoto, Kuboyama, Suzuki, Noda: "Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0119361, (2016) (PubMed).

    Sorensen, Conner: "γ-secretase-dependent cleavage initiates notch signaling from the plasma membrane." dans: Traffic (Copenhagen, Denmark), Vol. 11, Issue 9, pp. 1234-45, (2011) (PubMed).

  • Antigène
    Myc Tag
    Abstract
    Myc Tag Produits
    Classe de substances
    Tag
    Sujet
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
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