IL-27 anticorps

N° du produit ABIN1043835

Détail du produit anti-IL-27 anticorps

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-27 est non-conjugé

Application: Western Blotting (WB)

Specificité: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.

Attributs du produit: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.

Purification: purified

Stérilité: Sterile filtered

Immunogène: This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Immunogen Type: Recombinant Protein

Clone: 3H12-F10

Isotype: IgG2a kappa

Information d'application

Indications d'application: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Commentaires:

Gene Name: IL27

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-IL-27 (ABIN1043835)

Murugaiyan, Mittal, Weiner: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).

Détails sur IL-27

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: IL-27/p28 (IL27 Produits)

Synonymes: anticorps IL-27, anticorps IL-27A, anticorps IL27A, anticorps IL27p28, anticorps IL30, anticorps p28, anticorps RGD1561420, anticorps IL27, anticorps IL-27p28, anticorps Il30, anticorps interleukin 27, anticorps IL27, anticorps Il27

Sujet: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
Synonyms: Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

ID gène: 246779

NCBI Accession: NP_663611

UniProt: Q8K3I6