Nestin anticorps (AA 1484-1500)

N° du produit ABIN104564

Détail du produit anti-Nestin anticorps

Antigène: Nestin (NES)

Épitope: AA 1484-1500

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein.
Immunogentype:Recombinant

Isotype: IgG

Information d'application

Indications d'application: Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC) and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Références pour anticorps anti-Nestin (ABIN104564)

Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." dans: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." dans: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." dans: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin (NES Produits)

Synonymes: anticorps paranemin, anticorps NES, anticorps AA166324, anticorps C78523, anticorps ESTM46, anticorps nestin, anticorps nestin S homeolog, anticorps NES, anticorps nes.S, anticorps Nes, anticorps nes

Sujet: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Synonyms: nestin, ESTM 46 antibody, FLJ 21841 antibody, FLJ21841 antibody, Intermediate filament protein antibody

ID gène: 10763, 38176300

UniProt: P48681