Nestin anticorps (AA 1484-1500)

N° du produit ABIN104564

N° du produit (Fournisseur): 600-401-417

Détail du produit anti-Nestin anticorps

Antigène: Nestin (NES)

Épitope: AA 1484-1500

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nestin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

N° du produit (Fournisseur): 600-401-417

Fournisseur: Rockland

Fonction: Nestin Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Nestin protein.

Attributs du produit: Synonyms: rabbit anti-Nestin antibody, rabbit anti-NES antibody, Nbla00170

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Nestin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1484-1500 of human Nestin protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Affinity purified Anti-Nestin has been tested for use in ELISA, Immunohistochemistry (IHC), and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000 - 1:80,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store anti-Nestin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Nestin (ABIN104564)

Mokrý, Cízková, Filip, Ehrmann, Osterreicher, Kolár, English: "Nestin expression by newly formed human blood vessels." dans: Stem cells and development, Vol. 13, Issue 6, pp. 658-64, (2005) (PubMed).

Aihara, Sugawara, Torii, Hosaka, Kurihara, Saito, Takeuchi: "Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene." dans: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 12, pp. 1581-92, (2005) (PubMed).

Yaworsky, Kappen: "Heterogeneity of neural progenitor cells revealed by enhancers in the nestin gene." dans: Developmental biology, Vol. 205, Issue 2, pp. 309-21, (1999) (PubMed).

Dahlstrand, Zimmerman, McKay, Lendahl: "Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments." dans: Journal of cell science, Vol. 103 ( Pt 2), Issue 1, pp. 589-97, (1993) (PubMed).

Hockfield, McKay: "Identification of major cell classes in the developing mammalian nervous system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 5, Issue 12, pp. 3310-28, (1986) (PubMed).

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: NES (NES Produits)

Sujet: Background: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985).  Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube.  Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells.  Nestin expression is seen in almost all GBMs (Glioblastoma multiformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.  Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

ID gène: 10763, 38176300

UniProt: P48681