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5HT1B Receptor anticorps (Cytoplasmic Domain)

HTR1B Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache), Cobaye, Hamster, Roussette (Chauve-souris), Lapin, Porc IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048255
  • Antigène Voir toutes 5HT1B Receptor (HTR1B) Anticorps
    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))
    Épitope
    • 10
    • 7
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    Humain, Rat, Souris, Chien, Boeuf (Vache), Cobaye, Hamster, Roussette (Chauve-souris), Lapin, Porc
    Hôte
    • 35
    • 2
    • 1
    Lapin
    Clonalité
    • 37
    • 1
    Polyclonal
    Conjugué
    • 19
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp 5HT1B Receptor est non-conjugé
    Application
    • 28
    • 25
    • 8
    • 7
    • 3
    • 3
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human 5HT1B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Cat, Rabbit, Pig, Guinea pig (100%) Monkey, Horse, Opossum (94%) Chicken, Lizard, Xenopus (88%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human 5HT1B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Cat, Rabbit, Pig, Guinea pig (100%), Monkey, Horse, Opossum (94%), Chicken, Lizard, Xenopus (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HTR1B Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (7 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))
    Autre désignation
    HTR1B / 5-HT1B Receptor (HTR1B Produits)
    Synonymes
    anticorps 5-HT1B, anticorps 5-HT1DB, anticorps HTR1D2, anticorps HTR1DB, anticorps S12, anticorps 5-HT1Db, anticorps 5-HT1-LIKE, anticorps HTR1B, anticorps 5-HT-dro2B, anticorps 5-HT1BDro, anticorps 5-HT[1B]Dro, anticorps 5-HT[[1BDro]], anticorps 5-HT[[1B]], anticorps 5-HT[[1B]]DRO, anticorps 5-HT[[1B]]Dro, anticorps 5-HT[[dro2B]], anticorps 5HT-R2B, anticorps 5HT-dro2B, anticorps 5HT-dro[[2B]], anticorps 5HT1B, anticorps 5htdro2b, anticorps CG15113, anticorps CG30126, anticorps DRO2B, anticorps Dm5HTdro2B, anticorps Dmel\\CG15113, anticorps Dmel_CG30126, anticorps d5-HT1B, anticorps 5-HT-1B, anticorps zgc:194666, anticorps zgc:194690, anticorps 5-hydroxytryptamine receptor 1B, anticorps 5-hydroxytryptamine (serotonin) receptor 1B, anticorps HTR1B, anticorps Htr1b, anticorps 5-HT1B, anticorps htr1b
    Sujet
    Name/Gene ID: HTR1B
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1b receptor, 5-HT1DB, 5HT1B Receptor, HTR1DB, Serotonin 5-HT-1b receptor, Serotonin receptor 1B, Serotonin 1b receptor, HTR1D2, Serotonin 1D beta receptor
    ID gène
    3351
    Pathways
    Signalistation JAK/STAT, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins
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