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Serotonin Receptor 1E anticorps (Cytoplasmic Domain)

HTR1E Reactivité: Humain, Cheval, Boeuf (Vache), Cobaye, Singe, Poulet IHC (p), IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048261
  • Antigène Voir toutes Serotonin Receptor 1E (HTR1E) Anticorps
    Serotonin Receptor 1E (HTR1E)
    Épitope
    • 15
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 30
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Cheval, Boeuf (Vache), Cobaye, Singe, Poulet
    Hôte
    • 29
    • 1
    Lapin
    Clonalité
    • 29
    • 1
    Polyclonal
    Conjugué
    • 12
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Serotonin Receptor 1E est non-conjugé
    Application
    • 13
    • 13
    • 11
    • 8
    • 7
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
    Marque
    IHC-plus™
    Specificité
    Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HTR1F (65 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Horse, Guinea pig, Turkey, Sparrow, Chicken, Lizard (100%) Bat, Opossum (94%) Stickleback, Zebrafish (88%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 17 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Horse, Guinea pig, Turkey, Sparrow, Chicken, Lizard (100%), Bat, Opossum (94%), Stickleback, Zebrafish (88%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HTR1E Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (10 μg/mL)

    Usage: Antigen retrieval requires Proteinase K treatment.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    Serotonin Receptor 1E (HTR1E)
    Autre désignation
    HTR1E / 5-HT1E Receptor (HTR1E Produits)
    Synonymes
    anticorps Htr1ea, anticorps HTR1E, anticorps 5HT1E, anticorps 5-HT1E, anticorps 5-HT-1E, anticorps 5-hydroxytryptamine (serotonin) receptor 1E, anticorps 5-hydroxytryptamine receptor 1E, anticorps 5-hydroxytryptamine (serotonin) receptor 1E, G protein-coupled, anticorps Htr1e, anticorps HTR1E, anticorps htr1e
    Sujet
    Name/Gene ID: HTR1E
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR1E, 5-HT1E, 5-HT1e receptor, 5-HT-1E, 5HT1E Receptor, HT1e receptor, S31, Serotonin 5-HT-1e receptor, Serotonin 1e receptor, Serotonin receptor 1E
    ID gène
    3354
    Pathways
    Signalistation JAK/STAT, cAMP Metabolic Process
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