ADCYAP1R1 anticorps (N-Term)
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- Antigène Voir toutes ADCYAP1R1 Anticorps
- ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))
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Épitope
- N-Term
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Reactivité
- Humain, Boeuf (Vache), Singe
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ADCYAP1R1 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human ADCYAP1R1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%) Dog, Pig (94%) Mouse, Rat, Elephant, Panda (89%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 18 amino acid peptide from N-terminal extracellular domain of human ADCYAP1R1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%), Dog, Pig (94%), Mouse, Rat, Elephant, Panda (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ADCYAP1R1 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (5 μg/mL)
Usage: Antigen retrieval requires Proteinase K treatment. - Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- ADCYAP1R1 (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) Receptor Type I (ADCYAP1R1))
- Autre désignation
- ADCYAP1R1 / PAC1 Receptor (ADCYAP1R1 Produits)
- Synonymes
- anticorps PAC1, anticorps PAC1R, anticorps PACAPR, anticorps PACAPRI, anticorps 2900024I10Rik, anticorps AI846590, anticorps PACAP1-R, anticorps pac1, anticorps pacapr, anticorps pacapri, anticorps PAC1-R, anticorps PACAP-R1, anticorps PACAP-R1A, anticorps PACAPR1, anticorps PACAPR1A, anticorps adcyap1r1, anticorps adcyap1r1-A, anticorps ADCYAP1R1, anticorps ADCYAP receptor type I, anticorps adenylate cyclase activating polypeptide 1 receptor 1, anticorps adenylate cyclase activating polypeptide 1 (pituitary) receptor type I, anticorps ADCYAP receptor type 1, anticorps adenylate cyclase activating polypeptide 1a (pituitary) receptor type I, anticorps adenylate cyclase activating polypeptide 1 (pituitary) receptor type I S homeolog, anticorps ADCYAP1R1, anticorps Adcyap1r1, anticorps adcyap1r1, anticorps adcyap1r1a, anticorps adcyap1r1.S
- Sujet
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Name/Gene ID: ADCYAP1R1
Subfamily: Vasoactive intestinal polypeptide
Family: GPCR
Synonyms: ADCYAP1R1, PAC1, Pac1 receptor, Pac1 receptors, PAC1-R, PACAP receptor 1, Pacap specific receptor, PACAP-R-1, PACAP/VIP receptors, PACAP1 receptor, PACAP Receptor, Pacap receptor type 1, Pacap type 1 receptor, PACAP type I receptor, PACAPR, PAC1R, PACAP-R1, PACAPRI, Pvr1, Pacap receptor type i - ID gène
- 117
- Pathways
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
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