Angiotensin II Type 2 Receptor anticorps (Internal Region)
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- Antigène Voir toutes Angiotensin II Type 2 Receptor (AGTR2) Anticorps
- Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
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Épitope
- Internal Region
- Reactivité
- Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Chien, Singe, Roussette (Chauve-souris), Hamster, Mouton
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Angiotensin II Type 2 Receptor est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human AGTR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR65 (63 % ), NPFFR2 (44 %).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 16 amino acid peptide from internal region of human AGTR2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Pig (100%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product AGTR2 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Angiotensin II Type 2 Receptor (AGTR2) (Angiotensin II Receptor, Type 2 (AGTR2))
- Autre désignation
- AGTR2 / AT2 Receptor (AGTR2 Produits)
- Synonymes
- anticorps AT2, anticorps ATGR2, anticorps MRX88, anticorps AI316812, anticorps AW107640, anticorps AT2-R, anticorps AT2R, anticorps AT2R, anticorps at2, anticorps zgc:113016, anticorps AGTR2, anticorps angiotensin II receptor type 2, anticorps angiotensin II receptor, type 2, anticorps AGTR2, anticorps Agtr2, anticorps agtr2
- Sujet
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Name/Gene ID: AGTR2
Subfamily: Angiotensin
Family: GPCR
Synonyms: AGTR2, Angiotensin ii receptor type 2, Angiotensin II type-2 receptor, AT2 receptor, AT2, At2r, MRX88, Angiotensin ii at2, Angiotensin receptor 2, Type-2 angiotensin II receptor, ATGR2 - ID gène
- 186
- Pathways
- ACE Inhibitor Pathway, Hormone Transport, Regulation of Systemic Arterial Blood Pressure by Hormones
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