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C14orf1 anticorps (Internal Region)

C14orf1 Reactivité: Humain, Souris, Lapin, Singe, Roussette (Chauve-souris), Chien, Hamster, Rat, Boeuf (Vache), Porc IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048360
  • Antigène Tous les produits C14orf1
    C14orf1 (Chromosome 14 Open Reading Frame 1 (C14orf1))
    Épitope
    • 2
    • 1
    • 1
    Internal Region
    Reactivité
    Humain, Souris, Lapin, Singe, Roussette (Chauve-souris), Chien, Hamster, Rat, Boeuf (Vache), Porc
    Hôte
    • 4
    Lapin
    Clonalité
    • 4
    Polyclonal
    Conjugué
    • 4
    Cet anticorp C14orf1 est non-conjugé
    Application
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human C14orf1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except C14orf1 (100 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Platypus (100%) Horse, Guinea pig (92%) Salmon, Smelt, Stickleback, Medaka, Pufferfish (83%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 12 amino acid peptide from internal region of human C14orf1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Platypus (100%), Horse, Guinea pig (92%), Salmon, Smelt, Stickleback, Medaka, Pufferfish (83%).

    Type of Immunogen: Synthetic peptide
  • Indications d'application
    Approved: IHC, IHC-P (5 - 7.5 μg/mL)

    Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment using HIER with citrate buffer.
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    C14orf1 (Chromosome 14 Open Reading Frame 1 (C14orf1))
    Autre désignation
    C14orf1 (C14orf1 Produits)
    Synonymes
    anticorps ERG28, anticorps NET51, anticorps homolog of yeast ergosterol28, anticorps T27I1.5, anticorps T27I1_5, anticorps C7H14orf1, anticorps ergosterol biosynthesis 28 homolog, anticorps ergosterol biosynthetic protein 28, anticorps ergosterol biosynthesis protein, anticorps ERG28, anticorps LOC781060, anticorps ANI_1_490024, anticorps AOR_1_898014
    Sujet
    Name/Gene ID: C14orf1

    Synonyms: C14orf1, ERG28, NET51
    ID gène
    11161
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