CNR1 anticorps (C-Term)

N° du produit ABIN1048451

Détail du produit anti-CNR1 anticorps

Antigène: CNR1 (Cannabinoid Receptor 1 (CNR1))

Épitope: C-Term

Reactivité: Humain, Rat, Souris, Singe, Chien, Cheval, Boeuf (Vache), Roussette (Chauve-souris), Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CNR1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marque: IHC-plus™

Specificité: Human CNR1 / CB1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Dog, Bat, Bovine, Cat, Horse, Pig (100%) Opossum (94%) Turkey, Zebra finch, Chicken, Xenopus (88%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from C-terminus of human CNR1 / CB1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Panda, Dog, Bat, Bovine, Cat, Horse, Pig (100%), Opossum (94%), Turkey, Zebra finch, Chicken, Xenopus (88%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (5 - 8 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur CNR1

Antigène: CNR1 (Cannabinoid Receptor 1 (CNR1))

Autre désignation: CNR1 / CB1 (CNR1 Produits)

Synonymes: anticorps CANN6, anticorps CB-R, anticorps CB1, anticorps CB1A, anticorps CB1K5, anticorps CB1R, anticorps CNR, anticorps SKR6R, anticorps cnr1-A, anticorps cannabinoid receptor 1, anticorps cannabinoid receptor 1 (brain), anticorps cannabinoid receptor 1 (brain) S homeolog, anticorps CNR1, anticorps Cnr1, anticorps cnr1, anticorps cnr1.S

Sujet: Name/Gene ID: CNR1
Subfamily: Cannabinoid
Family: GPCR

Synonyms: CNR1, CB-R, CB1 receptor, CB1A, CB1R, Cannabinoid receptor 1, Cannabinoid receptor CB1, Cannabinoid Receptor I, CB1, CB1K5, CANN6, Cannabinoid receptor 1 (brain), CNR, Cannabinoid CB(1) receptor, CB1 cannabinoid receptor, Central cannabinoid receptor

ID gène: 1268

Pathways: Feeding Behaviour