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CXCR2 anticorps (Extracellular Domain)

CXCR2 Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048473
  • Antigène Voir toutes CXCR2 Anticorps
    CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))
    Épitope
    • 14
    • 13
    • 12
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 83
    • 40
    • 23
    • 1
    • 1
    • 1
    • 1
    Humain, Singe
    Hôte
    • 68
    • 18
    • 3
    • 1
    Lapin
    Clonalité
    • 70
    • 19
    • 1
    Polyclonal
    Conjugué
    • 44
    • 7
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CXCR2 est non-conjugé
    Application
    • 48
    • 30
    • 28
    • 22
    • 16
    • 15
    • 12
    • 8
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human CXCR2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Monkey (100%) Gorilla (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 15 amino acid peptide from 2nd extracellular domain of human CXCR2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Monkey (100%), Gorilla (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CXCR2 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))
    Autre désignation
    CXCR2 (CXCR2 Produits)
    Synonymes
    anticorps CD182, anticorps CDw128b, anticorps CMKAR2, anticorps IL8R2, anticorps IL8RA, anticorps IL8RB, anticorps CXC-R2, anticorps CXCR-2, anticorps CD128, anticorps CDw128, anticorps Cmkar2, anticorps Gpcr16, anticorps IL-8Rh, anticorps IL-8rb, anticorps Il8rb, anticorps mIL-8RH, anticorps C-X-C motif chemokine receptor 2, anticorps chemokine (C-X-C motif) receptor 2, anticorps Cxcr2, anticorps CXCR2
    Sujet
    Name/Gene ID: CXCR2
    Subfamily: Chemokine
    Family: GPCR

    Synonyms: CXCR2, CDw128b, CD182 antigen, CMKAR2, CXCR-2, CD182, CXC-R2, IL-8R B, Il-8 receptor b, IL-8 receptor type 2, Il-8rb, IL8R2, IL8RB, Interleukin-8 receptor type B, GRO/MGSA receptor, Interleukin 8 receptor beta, Interleukin 8 receptor, beta, Chemokine (CXC) receptor 2, IL8RA, Interleukin 8 receptor B, Interleukin 8 receptor type 2
    ID gène
    3579
    Pathways
    cAMP Metabolic Process
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