Dopamine Receptor d1 anticorps (Extracellular Domain)

N° du produit ABIN1048514

Détail du produit anti-Dopamine Receptor d1 anticorps

Antigène: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Épitope: Extracellular Domain

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Dopamine Receptor d1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human DRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Marmoset (95%) Elephant (90%) Dog, Rabbit (85%) Horse (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 20 amino acid peptide from 2nd extracellular domain of human DRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Marmoset (95%), Elephant (90%), Dog, Rabbit (85%), Horse (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL)

Not recommended for: ICC, IHC-Fr

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur Dopamine Receptor d1

Antigène: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Autre désignation: DRD1 / Dopamine Receptor D1 (DRD1 Produits)

Synonymes: anticorps DRD1, anticorps Dop1, anticorps D1A, anticorps DADR, anticorps DRD1A, anticorps drd1, anticorps C030036C15Rik, anticorps Drd-1, anticorps Drd1, anticorps Gpcr15, anticorps D1a, anticorps Drd1a, anticorps dopamine receptor D1, anticorps dopamine receptor, D1, anticorps dopamine receptor D1b, anticorps DRD1, anticorps Dop1, anticorps drd1b, anticorps Drd1

Sujet: Name/Gene ID: DRD1
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD1, D(1A) dopamine receptor, D1a dopamine receptor, Dopamine d-1 receptor, Dopamine receptor d1a, DRD1A, DADR, Dopamine d1a receptor, Dopamine receptor D1, Dopamine 1 receptor, Dopamine type 1 receptor, D-1 dopamine receptor, D1 dopamine receptor, D1a receptor, Dopamine D1 receptor

ID gène: 1812

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity