DUSP22 anticorps (N-Term)

N° du produit ABIN1048522

Détail du produit anti-DUSP22 anticorps

Antigène: DUSP22 (Dual Specificity Phosphatase 22 (DUSP22))

Épitope: N-Term

Reactivité: Humain, Lapin, Boeuf (Vache), Cheval, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DUSP22 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human DUSP22. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DUSP15 (59 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit (100%) Mouse, Rat (94%) Elephant, Chicken (88%) Xenopus (82%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 17 amino acid peptide from near N-terminus of human DUSP22. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Horse, Rabbit (100%), Mouse, Rat (94%), Elephant, Chicken (88%), Xenopus (82%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2 - 22 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur DUSP22

Antigène: DUSP22 (Dual Specificity Phosphatase 22 (DUSP22))

Autre désignation: DUSP22 / JSP 1 (DUSP22 Produits)

Synonymes: anticorps dusp22, anticorps wu:fa11e09, anticorps zgc:92816, anticorps vhx, anticorps jkap, anticorps jsp1, anticorps mkpx, anticorps DUSP22, anticorps ch73-170d1.1, anticorps zgc:112536, anticorps JKAP, anticorps JSP1, anticorps LMWDSP2, anticorps MKPX, anticorps VHX, anticorps 1110028K04Rik, anticorps AW212235, anticorps dual specificity phosphatase 22, anticorps dual specificity phosphatase 22a, anticorps dual specificity phosphatase 22b, anticorps DUSP22, anticorps dusp22a, anticorps dusp22, anticorps dusp22b, anticorps Dusp22

Sujet: Name/Gene ID: DUSP22
Subfamily: LMW-DSP
Family: Protein Phosphatase

Synonyms: DUSP22, JSP 1, JKAP, JNK-stimulating phosphatase 1, LMW-DSP2, Map kinase phosphatase x, MKPX, VHX, JNK-stimulatory phosphatase-1, JSP-1, JSP1, LMWDSP2, MKP-X

ID gène: 56940