Endothelin-1 Receptor anticorps (Extracellular Domain)
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- Antigène Voir toutes Endothelin-1 Receptor (EDNRA) Anticorps
- Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))
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Épitope
- Extracellular Domain
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Reactivité
- Humain, Souris, Chien, Boeuf (Vache), Porc, Cheval, Mouton, Lapin
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Endothelin-1 Receptor est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human Endothelin A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Sheep, Elephant, Panda, Bovine, Dog, Rabbit, Horse, Pig (100%) Marmoset, Rat, Hamster (94%) Bat (89%) Platypus (83%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 18 amino acid peptide from 2nd extracellular domain of human Endothelin A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Sheep, Elephant, Panda, Bovine, Dog, Rabbit, Horse, Pig (100%), Marmoset, Rat, Hamster (94%), Bat (89%), Platypus (83%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product EDNRA Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (5 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Endothelin-1 Receptor (EDNRA) (Endothelin Receptor Type A (EDNRA))
- Autre désignation
- EDNRA / Endothelin A Receptor (EDNRA Produits)
- Synonymes
- anticorps ET-A, anticorps ETA, anticorps ETA-R, anticorps ETAR, anticorps ETRA, anticorps hET-AR, anticorps ET-AR, anticorps Endor, anticorps Eta, anticorps RGD1559432, anticorps ETa, anticorps Gpcr10, anticorps ednr-a, anticorps ednra, anticorps wu:fb75g12, anticorps ednraa, anticorps eta, anticorps etar, anticorps etra, anticorps zgc:85942, anticorps endothelin receptor type A, anticorps endothelin receptor type Aa, anticorps endothelin receptor type A L homeolog, anticorps endothelin receptor type Ab, anticorps EDNRA, anticorps Ednra, anticorps ednraa, anticorps ednra.L, anticorps ednra, anticorps ednrab
- Sujet
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Name/Gene ID: EDNRA
Subfamily: Endothelin
Family: GPCR
Synonyms: EDNRA, Endothelin receptor subtype A, Et-a-r, ETRA, Endothelin A receptor, Endothelin eta receptor, Endothelin type a receptor, Endothelin-1 receptor, ETAR, Endothelin receptor type A, HET-AR, Et-a receptor, ETA, ETA-R, Endothelin receptor: subtype a, Endothelin-1-specific receptor, ET-A, ET-RA - ID gène
- 1909
- Pathways
- cAMP Metabolic Process
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