ESRRA anticorps (Internal Region)

N° du produit ABIN1048580

Détail du produit anti-ESRRA anticorps

Antigène: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Porc, Singe, Roussette (Chauve-souris), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ESRRA est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Marque: IHC-plus™

Specificité: Human ESRRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Pig (100%) Gibbon (93%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 15 amino acid peptide from internal region of human ESRRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Pig (100%), Gibbon (93%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ChrIP, IHC, IHC-P (8 - 24 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur ESRRA

Antigène: ESRRA (Estrogen-Related Receptor alpha (ESRRA))

Autre désignation: ESRRA / ERR alpha (ESRRA Produits)

Synonymes: anticorps err1, anticorps erra, anticorps esrral, anticorps erralpha, anticorps nr3b1, anticorps MGC80854, anticorps ESRRA, anticorps esrl1, anticorps LOC100125507, anticorps ERRalpha, anticorps Err1, anticorps Estrra, anticorps Nr3b1, anticorps Errra, anticorps ERR1, anticorps ERRa, anticorps ESRL1, anticorps NR3B1, anticorps ESTRRA, anticorps estrogen-related receptor alpha, anticorps estrogen related receptor alpha L homeolog, anticorps estrogen related receptor alpha, anticorps catsper channel auxiliary subunit zeta, anticorps estrogen related receptor, alpha, anticorps esrra, anticorps esrra.L, anticorps ESRRA, anticorps LOC100125507, anticorps CATSPERZ, anticorps Esrra

Sujet: Name/Gene ID: ESRRA
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESRRA, ERRa, ERR1, HERR1, NR3B1, Steroid hormone receptor ERR1, ERR-alpha, ERRalpha, ESRL1, Estrogen receptor-like 1

ID gène: 2101

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha