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FZD2 anticorps (N-Term)

FZD2 Reactivité: Humain, Rat, Souris, Boeuf (Vache), Chien, Cheval, Hamster, Porc, Singe, Lapin IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048612
  • Antigène Voir toutes FZD2 Anticorps
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    Épitope
    • 9
    • 8
    • 7
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    Humain, Rat, Souris, Boeuf (Vache), Chien, Cheval, Hamster, Porc, Singe, Lapin
    Hôte
    • 42
    • 2
    • 2
    • 1
    Lapin
    Clonalité
    • 44
    • 3
    Polyclonal
    Conjugué
    • 29
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp FZD2 est non-conjugé
    Application
    • 34
    • 23
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human Frizzled 2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD7 (60 % ), DMAP1 (40 %).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Platypus (100%) Opossum (95%) Turkey, Lizard, Newt (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 20 amino acid peptide from N-terminal extracellular domain of human Frizzled 2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Platypus (100%), Opossum (95%), Turkey, Lizard, Newt (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product FZD2 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (10 - 20 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    FZD2 (Frizzled Family Receptor 2 (FZD2))
    Autre désignation
    FSD2 / Frizzled 2 (FZD2 Produits)
    Synonymes
    anticorps BG01711, anticorps CG9739, anticorps D-Fz2, anticorps D-fz2, anticorps DFZ2, anticorps DFz-2, anticorps DFz2, anticorps Dfrz2, anticorps Dfz2, anticorps Dfz[[2]], anticorps Dm Fz2, anticorps Dmel\\CG9739, anticorps FZ2, anticorps Fz2, anticorps dFz2, anticorps dfz(2), anticorps dfz2, anticorps dfz2r, anticorps frz2, anticorps fz-2, anticorps fz8, anticorps zfz2, anticorps zg08, anticorps cb383, anticorps wu:fb70g09, anticorps FZD2, anticorps fzE2, anticorps hFz2, anticorps Fz-10, anticorps Fzd10, anticorps cFz-2, anticorps frizzled2, anticorps fz2, anticorps xfz2, anticorps AL033370, anticorps AW456835, anticorps Fz10, anticorps Mfz10, anticorps Mfz10a, anticorps frizzled 2, anticorps frizzled class receptor 2, anticorps frizzled class receptor 2 S homeolog, anticorps fz2, anticorps fzd2, anticorps FZD2, anticorps Fzd2, anticorps fzd2.S
    Sujet
    Name/Gene ID: FZD2
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2
    ID gène
    2535
    Pathways
    Signalisation WNT
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