FZD10 anticorps (N-Term)

N° du produit ABIN1048619

Détail du produit anti-FZD10 anticorps

Antigène: FZD10 (Frizzled Family Receptor 10 (FZD10))

Épitope: N-Term

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD10 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human FZD10 / Frizzled 10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Hamster, Panda, Dog (93%) Marmoset, Mouse, Bovine, Horse, Pig (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD10 / Frizzled 10. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Hamster, Panda, Dog (93%), Marmoset, Mouse, Bovine, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (6 - 16 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FZD10

Antigène: FZD10 (Frizzled Family Receptor 10 (FZD10))

Autre désignation: FZD10 / Frizzled 10 (FZD10 Produits)

Synonymes: anticorps CD350, anticorps FZ-10, anticorps Fz10, anticorps FzE7, anticorps hFz10, anticorps cFz-10, anticorps Fz-10, anticorps Xfr9, anticorps Xfz10, anticorps frizzled-10, anticorps frizzled10, anticorps fz10, anticorps fze7, anticorps hfz10, anticorps fk48e04, anticorps fz4, anticorps fzb, anticorps wu:fk48e04, anticorps zg04, anticorps Xfz10A, anticorps fzd10a, anticorps Xfz10B, anticorps Xfz9, anticorps fzd10b, anticorps fzd9, anticorps frizzled class receptor 10, anticorps frizzled class receptor 10 L homeolog, anticorps frizzled class receptor 10 S homeolog, anticorps FZD10, anticorps fzd10, anticorps Fzd10, anticorps fzd10.L, anticorps fzd10.S

Sujet: Name/Gene ID: FZD10
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD10, CD350 antigen, CD350, FZ-10, Frizzled homolog 10, HFz10, Frizzled 10, Frizzled family receptor 10, Frizzled-10, FzE7, Fz10

ID gène: 11211

Pathways: Signalisation WNT