FZD7 anticorps (N-Term)

N° du produit ABIN1048627

Détail du produit anti-FZD7 anticorps

Antigène: FZD7 (Frizzled Family Receptor 7 (FZD7))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Lapin, Roussette (Chauve-souris), Porc, Singe, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FZD7 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human FZD7 / Frizzled 7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD1 (60 % ), FZD2 (60 %).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum (100%) Xenopus (90%) Turkey, Chicken (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human FZD7 / Frizzled 7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Pig, Opossum (100%), Xenopus (90%), Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (4 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur FZD7

Antigène: FZD7 (Frizzled Family Receptor 7 (FZD7))

Autre désignation: FZD7 / Frizzled 7 (FZD7 Produits)

Synonymes: anticorps FzE3, anticorps Fz-7, anticorps Xfz7, anticorps frizzled-7, anticorps frizzled7, anticorps frz-7, anticorps frz7, anticorps fz7, anticorps fzd7-a, anticorps fzd7-b, anticorps fze3, anticorps Fz7, anticorps cFz-7, anticorps fb38g02, anticorps fc44b09, anticorps fz13, anticorps fz7a, anticorps fz7b, anticorps wu:fb38g02, anticorps wu:fc44b09, anticorps zg13, anticorps frizzled class receptor 7, anticorps frizzled class receptor 7 L homeolog, anticorps frizzled class receptor 7b, anticorps FZD7, anticorps fzd7.L, anticorps fzd7, anticorps Fzd7, anticorps fzd7b

Sujet: Name/Gene ID: FZD7
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD7, Frizzled family receptor 7, Frizzled homolog 7, Frizzled 7, Frizzled-7, FzE3, Fz-7, HFz7

ID gène: 8324

Pathways: Signalisation WNT, Stem Cell Maintenance