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G Protein-Coupled Receptor 182 anticorps (Cytoplasmic Domain)

GPR182 Reactivité: Humain IHC, IHC (p), ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1048768
  • Antigène Voir toutes G Protein-Coupled Receptor 182 (GPR182) Anticorps
    G Protein-Coupled Receptor 182 (GPR182)
    Épitope
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 25
    • 10
    • 3
    • 1
    Humain
    Hôte
    • 23
    • 2
    Lapin
    Clonalité
    • 23
    • 2
    Polyclonal
    Conjugué
    • 18
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp G Protein-Coupled Receptor 182 est non-conjugé
    Application
    • 11
    • 9
    • 8
    • 7
    • 4
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Marque
    IHC-plus™
    Specificité
    Human GPR182 / ADMR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset (90%) Pig (85%) Hamster, Bovine (80%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR182 / ADMR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset (90%), Pig (85%), Hamster, Bovine (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product GPR182 Anticorps primaire
  • Indications d'application
    Approved: ICC, IHC, IHC-P (5 - 9 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    G Protein-Coupled Receptor 182 (GPR182)
    Autre désignation
    GPR182 / ADMR (GPR182 Produits)
    Synonymes
    anticorps admr, anticorps fc05g10, anticorps wu:fc05g10, anticorps MGC69068, anticorps GPR182, anticorps ADMR, anticorps 7TMR, anticorps AM-R, anticorps AMR, anticorps G10D, anticorps gamrh, anticorps hrhAMR, anticorps Admr, anticorps G10-D, anticorps Gpcr17, anticorps Gpcr22, anticorps MB10, anticorps NOW, anticorps G protein-coupled receptor 182, anticorps G protein-coupled receptor 182 L homeolog, anticorps gpr182, anticorps gpr182.L, anticorps GPR182, anticorps Gpr182
    Sujet
    Name/Gene ID: GPR182
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: GPR182, 7TMR, Adrenomedullin L1 receptor, AM-R, AMR, ADMR, Adrenomedullin receptor, G protein-coupled receptor 182, HrhAMR, G-protein coupled receptor 182, Gamrh, HAMR, RAMR, G10D
    ID gène
    11318
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