G Protein-Coupled Receptor 182 anticorps (N-Term)

N° du produit ABIN1048769

Détail du produit anti-G Protein-Coupled Receptor 182 anticorps

Antigène: G Protein-Coupled Receptor 182 (GPR182)

Épitope: N-Term

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp G Protein-Coupled Receptor 182 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marque: IHC-plus™

Specificité: Human GPR182 / ADMR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset (100%) Gibbon (94%) Monkey (88%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from N-terminal extracellular domain of human GPR182 / ADMR. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset (100%), Gibbon (94%), Monkey (88%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ICC (8.5 - 17 μg/mL), IHC, IHC-P (17 μg/mL), WB

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur G Protein-Coupled Receptor 182

Antigène: G Protein-Coupled Receptor 182 (GPR182)

Autre désignation: GPR182 / ADMR (GPR182 Produits)

Synonymes: anticorps admr, anticorps fc05g10, anticorps wu:fc05g10, anticorps MGC69068, anticorps GPR182, anticorps ADMR, anticorps 7TMR, anticorps AM-R, anticorps AMR, anticorps G10D, anticorps gamrh, anticorps hrhAMR, anticorps Admr, anticorps G10-D, anticorps Gpcr17, anticorps Gpcr22, anticorps MB10, anticorps NOW, anticorps G protein-coupled receptor 182, anticorps G protein-coupled receptor 182 L homeolog, anticorps gpr182, anticorps gpr182.L, anticorps GPR182, anticorps Gpr182

Sujet: Name/Gene ID: GPR182
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR182, 7TMR, Adrenomedullin L1 receptor, AM-R, AMR, ADMR, Adrenomedullin receptor, G protein-coupled receptor 182, HrhAMR, G-protein coupled receptor 182, Gamrh, HAMR, RAMR, G10D

ID gène: 11318