Prostaglandin D2 Receptor 2 (PTGDR2) (Extracellular Domain) anticorps
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- Antigène Voir toutes Prostaglandin D2 Receptor 2 (PTGDR2) Anticorps
- Prostaglandin D2 Receptor 2 (PTGDR2)
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Épitope
- Extracellular Domain
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Reactivité
- Humain, Singe
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human GPR44 / CRTH2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%) Gorilla (97%) Panda, Dog, Pig (94%) Horse (91%) Elephant, Bovine (88%) Rabbit (85%) Mouse, Hamster (82%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 34 amino acid peptide from 2nd extracellular domain of human GPR44 / CRTH2. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%), Gorilla (97%), Panda, Dog, Pig (94%), Horse (91%), Elephant, Bovine (88%), Rabbit (85%), Mouse, Hamster (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PTGDR2 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (2.5 - 5 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Prostaglandin D2 Receptor 2 (PTGDR2)
- Autre désignation
- GPR44 / CRTH2 (PTGDR2 Produits)
- Synonymes
- anticorps CD294, anticorps CRTH2, anticorps DL1R, anticorps DP2, anticorps GPR44, anticorps Crth2, anticorps Grp45, anticorps Ptgdr2, anticorps Gpr44, anticorps prostaglandin D2 receptor 2, anticorps PTGDR2, anticorps Ptgdr2
- Sujet
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Name/Gene ID: PTGDR2
Subfamily: Prostanoid
Family: GPCR
Synonyms: PTGDR2, CD294, CRTH2, CD294 antigen, DP2, DL1R, G protein-coupled receptor 44, G-protein coupled receptor 44, HCRTH2, DP2 prostaglandin receptor, GPR44, Prostaglandin D2 receptor 2 - ID gène
- 11251
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
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