Leukotriene B4 Receptor/BLT anticorps (Cytoplasmic Domain)

N° du produit ABIN1049000

Détail du produit anti-Leukotriene B4 Receptor/BLT anticorps

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Épitope: Cytoplasmic Domain

Reactivité: Humain, Souris, Rat, Singe, Lapin, Hamster, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Leukotriene B4 Receptor/BLT est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human Leukotriene B4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%) Bovine (88%) Opossum, Platypus (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human Leukotriene B4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Horse, Rabbit (100%), Bovine (88%), Opossum, Platypus (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-Fr, IHC-P (5 - 10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-Leukotriene B4 Receptor/BLT (ABIN1049000)

Bäck, Bu, Bränström, Sheikine, Yan, Hansson: "Leukotriene B4 signaling through NF-kappaB-dependent BLT1 receptors on vascular smooth muscle cells in atherosclerosis and intimal hyperplasia." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 48, pp. 17501-6, (2005) (PubMed).

Détails sur Leukotriene B4 Receptor/BLT

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Autre désignation: Leukotriene B4 Receptor / BLT1 (LTB4R Produits)

Synonymes: anticorps BLT1, anticorps BLTR, anticorps CMKRL1, anticorps GPR16, anticorps LTB4R1, anticorps LTBR1, anticorps P2RY7, anticorps P2Y7, anticorps Ltb4r1, anticorps LTB4R, anticorps lt4r1, anticorps Ltb4r, anticorps mBLTR, anticorps leukotriene B4 receptor, anticorps leukotriene B4 receptor-like, anticorps Leukotriene B4 receptor 1, anticorps leukotriene B4 receptor 1, anticorps LTB4R, anticorps Ltb4r, anticorps LOC415617, anticorps lt4r1, anticorps Ltb4r1

Sujet: Name/Gene ID: LTB4R
Subfamily: Chemoattractant
Family: GPCR

Synonyms: LTB4R, BLT, BLT1, BLTR, Blt-1, Chemokine receptor-like 1, G protein-coupled receptor 16, GPR16, Leukotriene B4 receptor, Leukotriene B4 receptor 1, G-protein coupled receptor 16, LTBR1, p2Y purinoceptor 7, p2Y7, LTB4-R 1, p2RY7, CMKRL1, LTB4-R1, LTB4R1

ID gène: 1241