MRGPRX3 anticorps (Extracellular Domain)

N° du produit ABIN1049079

Détail du produit anti-MRGPRX3 anticorps

Antigène: MRGPRX3 (MAS-Related GPR, Member X3 (MRGPRX3))

Épitope: Extracellular Domain

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MRGPRX3 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marque: IHC-plus™

Specificité: Human MRGPRX3 / MRGX3. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RHCG (44 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gorilla (94%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from 3rd extracellular domain of human MRGPRX3 / MRGX3. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gorilla (94%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (16 - 32 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur MRGPRX3

Antigène: MRGPRX3 (MAS-Related GPR, Member X3 (MRGPRX3))

Autre désignation: MRGPRX3 / MRGX3 (MRGPRX3 Produits)

Synonymes: anticorps Gm660, anticorps Mrgprx3, anticorps GPCR, anticorps MRGX3, anticorps SNSR1, anticorps SNSR2, anticorps MrgA, anticorps Mrga10, anticorps Mrgpra, anticorps MAS-related GPR, member X3, pseudogene, anticorps MAS related GPR family member X3, anticorps mas-related G-protein coupled receptor member X2-like, anticorps MAS-related GPR, member X3, anticorps Mrgprx3-ps, anticorps MRGPRX3, anticorps LOC100359273, anticorps Mrgprx3

Sujet: Name/Gene ID: MRGPRX3
Subfamily: Orphan-A
Family: GPCR

Synonyms: MRGPRX3, MAS-related GPR, member X3, Hdrr1, HDRR2, MRGX3, SNSR1, SNSR2, Mas-related GPCR member X3

ID gène: 117195