TACR1 anticorps (C-Term)
-
- Antigène Voir toutes TACR1 Anticorps
- TACR1 (Tachykinin Receptor 1 (TACR1))
-
Épitope
- C-Term
-
Reactivité
- Humain, Rat, Cobaye, Singe, Lapin, Cheval, Hamster
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp TACR1 est non-conjugé
-
Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human Neurokinin 1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DTNB (44 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%) Mouse, Dog, Elephant (94%) Marmoset, Bat, Bovine (89%).
- Purification
- Immunoaffinity purified
- Immunogène
-
Synthetic 18 amino acid peptide from C-terminus of human Neurokinin 1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Rat, Hamster, Panda, Horse, Rabbit, Guinea pig (100%), Mouse, Dog, Elephant (94%), Marmoset, Bat, Bovine (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product TACR1 Anticorps primaire
-
-
- Indications d'application
- Approved: IHC, IHC-P (1 - 40 μg/mL)
- Commentaires
-
Target Species of Antibody: Human
- Procédure de l'essai
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
-
- Antigène
- TACR1 (Tachykinin Receptor 1 (TACR1))
- Autre désignation
- TACR1 / NK1R (TACR1 Produits)
- Synonymes
- anticorps NK1R, anticorps NKIR, anticorps SPR, anticorps TAC1R, anticorps Nk1r, anticorps Spr, anticorps Tac1r, anticorps nk1R, anticorps Tacr1, anticorps ASPR, anticorps tachykinin receptor 1, anticorps TACR1, anticorps Tacr1
- Sujet
-
Name/Gene ID: TACR1
Subfamily: Tachykinin
Family: GPCR
Synonyms: TACR1, Neurokinin receptor 1, NK-1R, Nk1 tachykinin receptor, NK1R, TAC1R, Tachykinin 1 receptor, NKIR, Substance-P receptor, Neurokinin 1 receptor, NK-1 receptor, Tachykinin receptor 1 - ID gène
- 6869
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
-