Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

TACR2 anticorps (Extracellular Domain)

TACR2 Reactivité: Humain, Singe IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049102
  • Antigène Voir toutes TACR2 Anticorps
    TACR2 (Tachykinin Receptor 2 (TACR2))
    Épitope
    • 15
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 24
    • 23
    • 21
    • 4
    • 3
    • 3
    Humain, Singe
    Hôte
    • 40
    • 1
    • 1
    Lapin
    Clonalité
    • 41
    • 1
    Polyclonal
    Conjugué
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TACR2 est non-conjugé
    Application
    • 29
    • 13
    • 13
    • 13
    • 9
    • 7
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marque
    IHC-plus™
    Specificité
    Human Neurokinin A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Bovine, Rabbit (94%) Horse, Pig (88%) Mouse, Rat, Bat, Hamster, Elephant, Guinea pig (81%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 16 amino acid peptide from 2nd extracellular domain of human Neurokinin A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Bovine, Rabbit (94%), Horse, Pig (88%), Mouse, Rat, Bat, Hamster, Elephant, Guinea pig (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product TACR2 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    TACR2 (Tachykinin Receptor 2 (TACR2))
    Autre désignation
    TACR2 / Nk2 / NK2R (TACR2 Produits)
    Synonymes
    anticorps NK-2, anticorps TACR2, anticorps NK2R, anticorps NKNAR, anticorps SKR, anticorps TAC2R, anticorps Tac2r, anticorps Nk2r, anticorps Skr, anticorps Rpa NK-2R, anticorps NK-2R, anticorps tachykinin receptor 2, anticorps TACR2, anticorps Tacr2
    Sujet
    Name/Gene ID: TACR2
    Subfamily: Tachykinin
    Family: GPCR

    Synonyms: TACR2, Neurokinin 2 receptor, NK-2 receptor, Neurokinin A receptor, NK2, NK2R, Tachykinin receptor 2, Substance-K receptor, NK-2R, SK receptor, SKR, TAC2R, NKNAR, Substance K receptor
    ID gène
    6865
    Pathways
    Hormone Transport, Negative Regulation of Hormone Secretion
Vous êtes ici:
Support technique