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NPBWR1 anticorps (Extracellular Domain)

NPBWR1 Reactivité: Humain, Rat IHC (p), IHC, IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049110
  • Antigène Voir toutes NPBWR1 Anticorps
    NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))
    Épitope
    • 15
    • 8
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivité
    • 40
    • 25
    • 10
    • 1
    Humain, Rat
    Hôte
    • 54
    • 1
    Lapin
    Clonalité
    • 54
    • 1
    Polyclonal
    Conjugué
    • 17
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NPBWR1 est non-conjugé
    Application
    • 32
    • 20
    • 13
    • 13
    • 10
    • 4
    • 3
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Marque
    IHC-plus™
    Specificité
    Human NPBWR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda (100%) Marmoset, Mouse, Rat, Bovine, Horse (95%) Rabbit (84%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 19 amino acid peptide from 2nd extracellular domain of human NPBWR1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Elephant, Panda (100%), Marmoset, Mouse, Rat, Bovine, Horse (95%), Rabbit (84%).

    Type of Immunogen: Synthetic peptide
  • Indications d'application
    Approved: IHC, IHC-Fr, IHC-P (6 - 12 μg/mL)
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Singh, Maguire, Kuc, Fidock, Davenport: "Identification and cellular localisation of NPW1 (GPR7) receptors for the novel neuropeptide W-23 by [125I]-NPW radioligand binding and immunocytochemistry." dans: Brain research, Vol. 1017, Issue 1-2, pp. 222-6, (2004) (PubMed).

  • Antigène
    NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))
    Autre désignation
    NPBWR1 / GPR7 (NPBWR1 Produits)
    Synonymes
    anticorps NPBWR1, anticorps GPR7, anticorps Gpr7, anticorps Nbpwr1, anticorps neuropeptides B/W receptor 1, anticorps neuropeptides B and W receptor 1, anticorps NPBWR1, anticorps Npbwr1
    Sujet
    Name/Gene ID: NPBWR1
    Subfamily: Neuropeptide
    Family: GPCR

    Synonyms: NPBWR1, G protein-coupled receptor 7, GPR7, Neuropeptide B/W receptor 1, Neuropeptides B/W receptor 1, G-protein coupled receptor 7
    ID gène
    2831
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