NR2E1 anticorps (N-Term)
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- Antigène Voir toutes NR2E1 Anticorps
- NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))
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Épitope
- N-Term
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Reactivité
- Humain, Lapin, Boeuf (Vache), Chien, Cheval, Porc, Roussette (Chauve-souris), Singe
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp NR2E1 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human NR2E1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%) Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).
- Purification
- Immunoaffinity purified
- Immunogène
- Synthetic 15 amino acid peptide from a N-terminal region of human NR2E1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Shrew, Bat, Bovine, Dog, Elephant, Panda, Rabbit, Horse, Pig, Opossum, Armadillo (100%), Mouse, Rat, Hamster, Sparrow, Chicken, Platypus, Lizard, Xenopus, Medaka, Pufferfish, Zebrafish, Stickleback (93%).
- Top Product
- Discover our top product NR2E1 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (5.5 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- NR2E1 (Nuclear Receptor Subfamily 2, Group E, Member 1 (NR2E1))
- Autre désignation
- NR2E1 / TLX (NR2E1 Produits)
- Synonymes
- anticorps NR2E1, anticorps TLL, anticorps TLX, anticorps XTLL, anticorps Mtl1, anticorps Mtll, anticorps Tlx, anticorps fierce, anticorps frc, anticorps tailless, anticorps fc30e03, anticorps fc39f12, anticorps wu:fc30e03, anticorps wu:fc39f12, anticorps zgc:100991, anticorps Xtll, anticorps nr2e1-A, anticorps tlx, anticorps nuclear receptor subfamily 2 group E member 1, anticorps nuclear receptor subfamily 2, group E, member 1, anticorps nuclear receptor subfamily 2 group E member 1 S homeolog, anticorps NR2E1, anticorps Nr2e1, anticorps nr2e1, anticorps nr2e1.S
- Sujet
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Name/Gene ID: NR2E1
Subfamily: NR2 Hepatocyte NF4-like
Family: NHR
Synonyms: NR2E1, HTll, Tailless homolog, Tailless, TLX, TLL, Nuclear receptor TLX, Protein tailless homolog, Tailes-related receptor, XTLL - ID gène
- 7101
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
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