P2RY6 anticorps (Cytoplasmic Domain)
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- Antigène Voir toutes P2RY6 Anticorps
- P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))
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Épitope
- Cytoplasmic Domain
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Reactivité
- Humain, Rat, Lapin, Boeuf (Vache), Porc, Singe, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp P2RY6 est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human P2RY6 / P2Y6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MOGAT1 (47 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Bovine, Rabbit, Pig (100%) Mouse, Panda, Dog, Bat (94%) Elephant, Pufferfish (82%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 17 amino acid peptide from 2nd cytoplasmic domain of human P2RY6 / P2Y6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Bovine, Rabbit, Pig (100%), Mouse, Panda, Dog, Bat (94%), Elephant, Pufferfish (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product P2RY6 Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (17 - 34 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- P2RY6 (Pyrimidinergic Receptor P2Y, G-Protein Coupled, 6 (P2RY6))
- Autre désignation
- P2RY6 / P2Y6 (P2RY6 Produits)
- Synonymes
- anticorps P2Y6, anticorps 2010204J23Rik, anticorps P2y6, anticorps P2RY3, anticorps P2Y3, anticorps P2Y6R, anticorps pyrimidinergic receptor P2Y6, anticorps pyrimidinergic receptor P2Y, G-protein coupled, 6, anticorps P2RY6, anticorps P2ry6
- Sujet
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Name/Gene ID: P2RY6
Subfamily: Purinergic
Family: GPCR
Synonyms: P2RY6, G-coupled nucleotide receptor, p2 purinoceptor, p2y3 receptor, p2Y purinoceptor 6, Purinergic receptor p2y6, p2RY6, p2y3 and chick, p2Y6 receptor, p2Y6, Purinoceptor p2y6 - ID gène
- 5031
- Pathways
- Smooth Muscle Cell Migration
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