PTPRA anticorps (Cytoplasmic Domain)
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- Antigène Voir toutes PTPRA Anticorps
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
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Épitope
- Cytoplasmic Domain
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Reactivité
- Humain, Souris, Chien, Boeuf (Vache), Singe, Porc, Lapin, Cheval, Roussette (Chauve-souris), Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PTPRA est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat (94%) Elephant, Turkey, Chicken (88%) Xenopus (82%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 17 amino acid peptide from cytoplasmic domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%), Rat (94%), Elephant, Turkey, Chicken (88%), Xenopus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PTPRA Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (10 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
- Autre désignation
- PTPRA / RPTP-alpha (PTPRA Produits)
- Synonymes
- anticorps PTPRA, anticorps heptp, anticorps hlpr, anticorps hptpa, anticorps hptpalpha, anticorps lrp, anticorps ptpa, anticorps ptprl2, anticorps r-ptp-alpha, anticorps rptpa, anticorps HEPTP, anticorps HLPR, anticorps HPTPA, anticorps HPTPalpha, anticorps LRP, anticorps PTPA, anticorps PTPRL2, anticorps R-PTP-alpha, anticorps RPTPA, anticorps Ptpa, anticorps Ptpalpha, anticorps Rptpalpha, anticorps Rptra, anticorps Rptralpha, anticorps RPTP[a], anticorps zf-RPTP[a], anticorps ptpa-a, anticorps protein tyrosine phosphatase, receptor type A, anticorps protein tyrosine phosphatase, receptor type, A, anticorps protein tyrosine phosphatase, receptor type A S homeolog, anticorps PTPRA, anticorps ptpra, anticorps Ptpra, anticorps ptpra.S
- Sujet
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Name/Gene ID: PTPRA
Subfamily: R4
Family: Protein Phosphatase
Synonyms: PTPRA, HLPR, Hptp alpha, HEPTP, HPTPA, PTPA, Ptpalpha, PTPRL2, PTP alpha, PTPLCA-related phosphatase, R-PTP-alpha, RPTPA, Rptpalpha, Tyrosine phosphatase alpha, RPTP alpha, HPTPalpha, LRP, PTPase-alpha - ID gène
- 5786
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