PTPRA anticorps (Extracellular Domain)
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- Antigène Voir toutes PTPRA Anticorps
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
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Épitope
- Extracellular Domain
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PTPRA est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human PTPRA. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except HLA-S (59 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Rat (94%) Hamster, Dog, Bovine, Rabbit (88%) Mouse, Panda, Horse (82%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 17 amino acid peptide from extracellular domain of human PTPRA. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat (94%), Hamster, Dog, Bovine, Rabbit (88%), Mouse, Panda, Horse (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PTPRA Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-P (15 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL. - Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- PTPRA (Protein tyrosine Phosphatase, Receptor Type, A (PTPRA))
- Autre désignation
- PTPRA / RPTP-alpha (PTPRA Produits)
- Synonymes
- anticorps PTPRA, anticorps heptp, anticorps hlpr, anticorps hptpa, anticorps hptpalpha, anticorps lrp, anticorps ptpa, anticorps ptprl2, anticorps r-ptp-alpha, anticorps rptpa, anticorps HEPTP, anticorps HLPR, anticorps HPTPA, anticorps HPTPalpha, anticorps LRP, anticorps PTPA, anticorps PTPRL2, anticorps R-PTP-alpha, anticorps RPTPA, anticorps Ptpa, anticorps Ptpalpha, anticorps Rptpalpha, anticorps Rptra, anticorps Rptralpha, anticorps RPTP[a], anticorps zf-RPTP[a], anticorps ptpa-a, anticorps protein tyrosine phosphatase, receptor type A, anticorps protein tyrosine phosphatase, receptor type, A, anticorps protein tyrosine phosphatase, receptor type A S homeolog, anticorps PTPRA, anticorps ptpra, anticorps Ptpra, anticorps ptpra.S
- Sujet
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Name/Gene ID: PTPRA
Subfamily: R4
Family: Protein Phosphatase
Synonyms: PTPRA, HLPR, Hptp alpha, HEPTP, HPTPA, PTPA, Ptpalpha, PTPRL2, PTP alpha, PTPLCA-related phosphatase, R-PTP-alpha, RPTPA, Rptpalpha, Tyrosine phosphatase alpha, RPTP alpha, HPTPalpha, LRP, PTPase-alpha - ID gène
- 5786
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