RORA anticorps (Hinge Region)
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- Antigène Voir toutes RORA Anticorps
- RORA (RAR-Related Orphan Receptor A (RORA))
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Épitope
- Hinge Region
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Singe, Porc, Roussette (Chauve-souris), Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp RORA est non-conjugé
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human ROR Alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RORB (65 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%) Opossum, Turkey, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish, Stickleback (95%).
- Purification
- Immunoaffinity purified
- Immunogène
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Synthetic 20 amino acid peptide from hinge domain of human ROR Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%), Opossum, Turkey, Chicken, Platypus, Xenopus, Pufferfish, Zebrafish, Stickleback (95%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product RORA Anticorps primaire
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- Indications d'application
- Approved: IHC, IHC-P (11 - 17 μg/mL)
- Commentaires
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Target Species of Antibody: Human
- Procédure de l'essai
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- RORA (RAR-Related Orphan Receptor A (RORA))
- Autre désignation
- RORA / ROR alpha (RORA Produits)
- Synonymes
- anticorps ror1, anticorps ror2, anticorps ror3, anticorps rzra, anticorps nr1f1, anticorps MGC146531, anticorps NR1F1, anticorps ROR1, anticorps ROR2, anticorps ROR3, anticorps RZR-ALPHA, anticorps RZRA, anticorps RORalpha-B, anticorps gb:dq017624, anticorps rora2, anticorps 9530021D13Rik, anticorps Nr1f1, anticorps nmf267, anticorps sg, anticorps staggerer, anticorps tmgc26, anticorps RORalpha1, anticorps RAR related orphan receptor A, anticorps RAR-related orphan receptor A, anticorps RAR-related orphan receptor A, paralog a, anticorps RAR-related orphan receptor alpha, anticorps RORA, anticorps rora, anticorps Rora, anticorps roraa
- Sujet
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Name/Gene ID: RORA
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: RORA, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Roralpha, Transcription factor RZR-alpha, RAR-related orphan receptor A, ROR-alpha, ROR3, RZRA, NR1F1, ROR Alpha, RZR-ALPHA - ID gène
- 6095
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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