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RXFP2 anticorps (Cytoplasmic Domain)

RXFP2 Reactivité: Humain, Souris, Cheval WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN1049309
  • Antigène Voir toutes RXFP2 Anticorps
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Épitope
    • 14
    • 11
    • 8
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    Reactivité
    • 49
    • 13
    • 8
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain, Souris, Cheval
    Hôte
    • 51
    Lapin
    Clonalité
    • 51
    Polyclonal
    Conjugué
    • 26
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RXFP2 est non-conjugé
    Application
    • 28
    • 23
    • 6
    • 6
    • 5
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificité
    Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
     Réactivité croisée
    Cheval
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Monkey, Panda (93%) Dog, Pig, Xenopus (86%).
    Purification
    Immunoaffinity purified
    Immunogène
    Synthetic 14 amino acid peptide from 2nd cytoplasmic domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Monkey, Panda (93%), Dog, Pig, Xenopus (86%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product RXFP2 Anticorps primaire
  • Indications d'application
    Approved: IHC, IHC-P (5 - 10 μg/mL), WB
    Commentaires

    Target Species of Antibody: Human

    Procédure de l'essai

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Autre désignation
    RXFP2 / LGR8 (RXFP2 Produits)
    Synonymes
    anticorps GPR106, anticorps GREAT, anticorps INSL3R, anticorps LGR8, anticorps LGR8.1, anticorps RXFPR2, anticorps Gpcr, anticorps Lgr8, anticorps Gpr106, anticorps Great, anticorps lgr8.1, anticorps rxfp2, anticorps rxfp2b, anticorps zgc:63668, anticorps relaxin/insulin like family peptide receptor 2, anticorps relaxin/insulin-like family peptide receptor 2, anticorps relaxin/insulin-like family peptide receptor 2a, anticorps RXFP2, anticorps Rxfp2, anticorps rxfp2a
    Sujet
    Name/Gene ID: RXFP2
    Subfamily: Relaxin
    Family: GPCR

    Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2
    ID gène
    122042
    Pathways
    cAMP Metabolic Process, Myometrial Relaxation and Contraction
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