SLC7A5 anticorps (Internal Region)
-
- Antigène Voir toutes SLC7A5 Anticorps
- SLC7A5 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5))
-
Épitope
- Internal Region
-
Reactivité
- Humain, Chien, Singe
-
Hôte
- Lapin
-
Clonalité
- Polyclonal
-
Conjugué
- Cet anticorp SLC7A5 est non-conjugé
-
Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marque
- IHC-plus™
- Specificité
- Human SLC7A5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Monkey, Marmoset, Dog (100%) Human, Panda, Bat, Rabbit (93%) Elephant, Bovine, Horse (87%).
- Purification
- Immunoaffinity purified
- Immunogène
-
Synthetic 15 amino acid peptide from internal region of human SLC7A5. Percent identity with other species by BLAST analysis: Monkey, Marmoset, Dog (100%), Human, Panda, Bat, Rabbit (93%), Elephant, Bovine, Horse (87%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC7A5 Anticorps primaire
-
-
- Indications d'application
- Approved: IHC, IHC-P (7 μg/mL)
- Commentaires
-
Target Species of Antibody: Human
- Procédure de l'essai
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Date de péremption
- 12 months
-
- Antigène
- SLC7A5 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5))
- Autre désignation
- SLC7A5 / CD98 Light Chain (SLC7A5 Produits)
- Synonymes
- anticorps 4F2LC, anticorps CD98, anticorps D16S469E, anticorps E16, anticorps LAT1, anticorps MPE16, anticorps hLAT1, anticorps D0H16S474E, anticorps TA1, anticorps JHI-21, anticorps SLC7A5, anticorps LAT-1, anticorps slc7a5a, anticorps 4F2, anticorps 4F2HC, anticorps AI314110, anticorps Cd98, anticorps Ly-10, anticorps Ly-m10, anticorps Ly10, anticorps Mdu1, anticorps Mgp-2hc, anticorps NACAE, anticorps solute carrier family 7 member 5, anticorps solute carrier family 7 (cationic amino acid transporter, y+ system), member 5, anticorps solute carrier family 7 (amino acid transporter light chain, L system), member 5, anticorps solute carrier family 7 member 5 S homeolog, anticorps solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2, anticorps SLC7A5, anticorps Slc7a5, anticorps slc7a5.S, anticorps Slc3a2
- Sujet
-
Name/Gene ID: SLC7A5
Subfamily: Cationic amino acid transporter
Family: Transporter
Synonyms: SLC7A5, 4F2 LC, 4F2 light chain, 4F2LC, CD98, CD98 light chain, CD98LC, D16S469E, Integral membrane protein E16, Membrane protein E16, MPE16, LAT1, E16, HLAT1, TA1 - ID gène
- 8140
-