STX1A anticorps (N-Term)

N° du produit ABIN1049369

Détail du produit anti-STX1A anticorps

Antigène: STX1A (Syntaxin 1A (Brain) (STX1A))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Singe, Porc, Hamster, Xenopus laevis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STX1A est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except STX2 (67 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%) Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%) Elephant (87%) Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 15 amino acid peptide from N-Terminus of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%), Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%), Elephant (87%), Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur STX1A

Antigène: STX1A (Syntaxin 1A (Brain) (STX1A))

Autre désignation: STX1A / Syntaxin 1A (STX1A Produits)

Synonymes: anticorps HPC-1, anticorps P35-1, anticorps STX1, anticorps SYN1A, anticorps STX1A, anticorps hpc-1, anticorps p35-1, anticorps stx1, anticorps sx1a, anticorps syntaxin1a, anticorps CG10716, anticorps CG18615, anticorps CG31136, anticorps CG5448, anticorps CT30033, anticorps Dm Syx1, anticorps DmSyx1A, anticorps Dmel\\CG31136, anticorps SYX1A, anticorps Syt1A, anticorps Syx, anticorps Syx-1A, anticorps Syx1, anticorps Syx1a, anticorps SyxA, anticorps anon-EST:Gibbs4, anticorps anon-WO02059370.54, anticorps dSyx1, anticorps l(3)06737, anticorps synt, anticorps syt-1A, anticorps syx, anticorps syx-1, anticorps syx-1A, anticorps syx1, anticorps syx1A, anticorps syntaxin 1A, anticorps syntaxin 1A (brain), anticorps Syntaxin 1A, anticorps syntaxin-1A, anticorps STX1A, anticorps Stx1a, anticorps stx1a, anticorps Syx1A, anticorps EDI_005240, anticorps Tsp_07354

Sujet: Name/Gene ID: STX1A
Family: Syntaxin/epimorphin

Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1

ID gène: 6804

Pathways: Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport