WNK1 anticorps (C-Term)

N° du produit ABIN1049464

Détail du produit anti-WNK1 anticorps

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Singe, Lapin, Cheval, Roussette (Chauve-souris), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WNK1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human WNK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Opossum (100%) Pig (94%) Bovine (88%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 17 amino acid peptide from near C-terminus of human WNK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Opossum (100%), Pig (94%), Bovine (88%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 - 7.5 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur WNK1

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Autre désignation: WNK1 / p65 (WNK1 Produits)

Synonymes: anticorps HSAN2, anticorps HSN2, anticorps KDP, anticorps PRKWNK1, anticorps PSK, anticorps p65, anticorps WNK1, anticorps kdp, anticorps psk, anticorps hsn2, anticorps hsan2, anticorps pha2c, anticorps prkwnk1, anticorps 6430573H23Rik, anticorps EG406236, anticorps Hsn2, anticorps Prkwnk1, anticorps mKIAA0344, anticorps ATWNK1, anticorps T9J14.14, anticorps T9J14_14, anticorps ZIK4, anticorps with no lysine (K) kinase 1, anticorps WNK lysine deficient protein kinase 1, anticorps WNK lysine deficient protein kinase 1 L homeolog, anticorps serine/threonine-protein kinase WNK1, anticorps with no lysine (K) kinase 1, anticorps WNK1, anticorps wnk1.L, anticorps wnk1, anticorps LOC100056745, anticorps Wnk1

Sujet: Name/Gene ID: WNK1
Subfamily: WNK
Family: Protein Kinase

Synonyms: WNK1, Erythrocyte 65 kDa protein, HSAN2, KIAA0344, Kinase deficient protein, HWNK1, PHA2C, PRKWNK1, PSK, HSN2, KDP

ID gène: 65125