xpr1 anticorps (C-Term)

N° du produit ABIN1049493

Détail du produit anti-xpr1 anticorps

Antigène: xpr1 (Xenotropic and Polytropic Retrovirus Receptor 1 (xpr1))

Épitope: C-Term

Reactivité: Humain, Souris, Hamster, Singe, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp xpr1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marque: IHC-plus™

Specificité: Human XPR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Cat, Bovine, Dog, Elephant, Horse, Pig (94%) Panda, Armadillo (88%) Bat, Turkey, Chicken, Platypus (81%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 16 amino acid peptide from C-terminus of human XPR1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Cat, Bovine, Dog, Elephant, Horse, Pig (94%), Panda, Armadillo (88%), Bat, Turkey, Chicken, Platypus (81%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (8 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur xpr1

Antigène: xpr1 (Xenotropic and Polytropic Retrovirus Receptor 1 (xpr1))

Autre désignation: XPR1 (xpr1 Produits)

Synonymes: anticorps xpr1, anticorps MGC80178, anticorps XPR1, anticorps SYG1, anticorps X3, anticorps Rmc-1, anticorps Rmc1, anticorps Sxv, anticorps Syg1, anticorps XR, anticorps xenotropic and polytropic retrovirus receptor 1, anticorps xenotropic and polytropic retrovirus receptor 1 L homeolog, anticorps similar to Xpr1-prov protein, anticorps KIAA1614 ortholog, anticorps XPR1, anticorps xpr1.L, anticorps xpr1, anticorps LOC562352, anticorps KIAA1614, anticorps Xpr1

Sujet: Name/Gene ID: XPR1
Subfamily: Orphan-U
Family: GPCR

Synonyms: XPR1, XR, SYG1, X3, Protein SYG1 homolog, X-receptor

ID gène: 9213