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NCKAP1 anticorps (Internal Region)

Verified NCKAP1 Reactivité: Humain ELISA, IF, FACS Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN1049510
  • Antigène Voir toutes NCKAP1 Anticorps
    NCKAP1 (NCK Associated Protein 1 (NCKAP1))
    Épitope
    • 15
    • 7
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 32
    • 27
    • 11
    Humain
    Hôte
    • 47
    Chèvre
    Clonalité
    • 47
    Polyclonal
    Conjugué
    • 16
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NCKAP1 est non-conjugé
    Application
    • 37
    • 17
    • 3
    • 2
    • 2
    • 2
    • 1
    ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Fonction
    NCKAP1
    Séquence
    TPREYLTSHL EIR
    Specificité
    This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).
     Réactivité croisée
    Boeuf (Vache), Chien, Humain, Souris, Porc, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Immunogène
    Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.
    Isotype
    IgG
    Top Product
    Discover our top product NCKAP1 Anticorps primaire
  • Indications d'application

    Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    NCKAP1 (NCK Associated Protein 1 (NCKAP1))
    Autre désignation
    NCKAP1 (NCKAP1 Produits)
    Synonymes
    anticorps HEM2, anticorps NAP1, anticorps NAP125, anticorps p125Nap1, anticorps C79304, anticorps H19, anticorps Hem-2, anticorps Hem2, anticorps Nap1, anticorps mKIAA0587, anticorps mh19, anticorps NCK associated protein 1, anticorps NCK-associated protein 1, anticorps NCK-associated protein 1 L homeolog, anticorps NCKAP1, anticorps Nckap1, anticorps nckap1.L
    Sujet
    NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1
    ID gène
    10787, 50884, 58823
    NCBI Accession
    NP_038464, NP_995314
    Pathways
    Signalisation RTK, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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