PPARA anticorps (N-Term)

N° du produit ABIN105798

Détail du produit anti-PPARA anticorps

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Épitope: N-Term

Reactivité: Souris, Rat, Chien, Boeuf (Vache), Porc, Hamsters dorés syriens

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPARA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: This affinity purified antibody is directed against mouse PPAR alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.

Attributs du produit: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Purification: affinity purified

Stérilité: Sterile filtered

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1 to 18 of mouse PPAR alpha.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for IHC. Specific conditions for reactivity should be optimized by the end user.

Commentaires:

Gene Name: PPARA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Références pour anticorps anti-PPARA (ABIN105798)

Zak, Gelebart, Lai: "Fenofibrate induces effective apoptosis in mantle cell lymphoma by inhibiting the TNFalpha/NF-kappaB signaling axis." dans: Leukemia, Vol. 24, Issue 8, pp. 1476-86, (2010) (PubMed).

Plutzky: "Medicine. PPARs as therapeutic targets: reverse cardiology?" dans: Science (New York, N.Y.), Vol. 302, Issue 5644, pp. 406-7, (2003) (PubMed).

Mukherjee, Jow, Noonan, McDonnell: "Human and rat peroxisome proliferator activated receptors (PPARs) demonstrate similar tissue distribution but different responsiveness to PPAR activators." dans: The Journal of steroid biochemistry and molecular biology, Vol. 51, Issue 3-4, pp. 157-66, (1995) (PubMed).

Détails sur PPARA

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Autre désignation: PPAR alpha (PPARA Produits)

Synonymes: anticorps NR1C1, anticorps PPAR, anticorps PPARalpha, anticorps hPPAR, anticorps LOC100136415, anticorps PPARALPHA, anticorps nr1c1, anticorps xPPARalpha, anticorps xppara, anticorps 4933429D07Rik, anticorps AW742785, anticorps Nr1c1, anticorps PPAR-alpha, anticorps Ppar, anticorps peroxisome proliferator activated receptor alpha, anticorps peroxisome proliferator-activated receptor alpha, anticorps peroxisome proliferator activated receptor alpha L homeolog, anticorps PPARA, anticorps LOC100136415, anticorps ppara.L, anticorps Ppara, anticorps ppara

Sujet: Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and Anti diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Synonyms: hPPAR antibody, MGC2237 antibody, MGC2452 antibody, NR1C1 antibody, Nuclear receptor subfamily 1 group C member 1 antibody, Peroxisome Proliferator Activated Receptor alpha antibody, PPAR antibody

ID gène: 19013, 31543500

UniProt: P23204

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C