NEDD8 anticorps

N° du produit ABIN106594

Détail du produit anti-NEDD8 anticorps

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NEDD8 est non-conjugé

Application: Western Blotting (WB)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Immunogentype:Recombinant

Isotype: IgG

Information d'application

Indications d'application: Rub1 Antibody is deected as a 6 kDa band corresponding to yeast Rub1in immunoblotting.  Most yeast cell lysates can be used as a positive control without induction or stimulation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-NEDD8 (ABIN106594)

Toro, Toth, Petroski: "The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics." dans: The Journal of biological chemistry, Vol. 288, Issue 21, pp. 14716-26, (2013) (PubMed).

Détails sur NEDD8

Antigène: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Autre désignation: Rub1 (NEDD8 Produits)

Synonymes: anticorps NEDD-8, anticorps Rub1, anticorps CG10679, anticorps Dmel\\CG10679, anticorps NEDD8, anticorps dNEDD8, anticorps nedd8, anticorps ubiquitin, anticorps si:zc14a17.5, anticorps GB17785, anticorps DDBDRAFT_0218116, anticorps DDBDRAFT_0238041, anticorps DDB_0218116, anticorps DDB_0238041, anticorps nedd8.S, anticorps neural precursor cell expressed, developmentally down-regulated 8, anticorps neural precursor cell expressed, developmentally down-regulated gene 8, anticorps CG10679 gene product from transcript CG10679-RB, anticorps Nedd8, anticorps ubiquitin-like protein Nedd8, anticorps neural precursor cell expressed, developmentally down-regulated 8 L homeolog, anticorps NEDD8, anticorps Nedd8, anticorps nedd8, anticorps nedd8.L

Sujet: Ubiquitin-like proteins fall into  two classes: the first class, ubiquitin-like modifiers (UBL’s) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBL’s are SUMO, Rub1 (also called Nedd8), Apg12, and Hub1.  Proteins of the second class include parkin, RAD23, and DSK2, and are designated ubiquitin-domain proteins (UDP’s). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBL’s, UDP’s are not proteolytically processed or conjugated to other proteins.  Rub1, and the corresponding human homolog Nedd8, are activated by the E1 ubiquitin-activating enzyme UBA2, which forms isopeptide linkages between thioesters.  Nedd8 shows 80% homology to ubiquitin.  The best known targets of Rub1 modification are members of the cullin family.  Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1/Cul1/Cdc53-F-box (SCF).  The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein.  Rub1 modification may regulate SCF function or localization.
Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1

ID gène: 851717

UniProt: Q03919

Pathways: Ubiquitin Proteasome Pathway