SUMO anticorps

N° du produit ABIN107152

Détail du produit anti-SUMO anticorps

Antigène: SUMO

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SUMO est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human SUMO protein.
Immunogentype:Recombinant

Isotype: IgG

Information d'application

Indications d'application: This purified polyclonal antibody reacts with human SUMO by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 1:2,000 dilution is recommended.  An 11.6 kDa band corresponding to human SUMO is detected.  Most human cell lysates can be used as a positive control without induction or stimulation.  For ELISA a 1:4,000 to 1:20,000 dilution is recommended.   Researchers should determine optimal titers for other applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-SUMO (ABIN107152)

Meyer, Shah, Zhang, Rohrs, Rao: "Evidence for intermolecular interactions between the intracellular domains of the arabidopsis receptor-like kinase ACR4, its homologs and the Wox5 transcription factor." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0118861, (2016) (PubMed).

Détails sur SUMO

Antigène: SUMO

Abstract: SUMO Produits

Synonymes: anticorps CG4494, anticorps Dm0342, anticorps DmSUMO-1, anticorps DmSmt3, anticorps Dmel\\CG4494, anticorps Dmsmt3, anticorps SMT3, anticorps SUMO, anticorps Smt3, anticorps Sumo, anticorps anon-EST:Posey240, anticorps dSmt3, anticorps dsmt3, anticorps l(2)04493, anticorps l(2)SH0182, anticorps l(2)SH2 0182, anticorps sumo, anticorps CG4494 gene product from transcript CG4494-RA, anticorps smt3

Sujet: Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression.  But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization.  Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein.  Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans.   Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in
Synonyms: GAP modifying protein 1 antibody, GMP 1 antibody, GMP1 antibody, PIC 1 antibody, PIC1 antibody, SENP2 antibody, Sentrin 1 antibody, Sentrin antibody, Small ubiquitin related modifier 1 antibody

ID gène: 7341

UniProt: P63165